黑曲霉產(chǎn)酸性蛋白酶催化機(jī)制和穩(wěn)定劑研究.pdf

1、Y768963學(xué)校編號(hào)：!鮑塑學(xué)號(hào)：2嫂蟄塑圖書分類號(hào)：——福建師范大學(xué)碩士學(xué)位論文黑曲霉產(chǎn)酸性蛋白酶催化機(jī)制和穩(wěn)定劑研究StudiesonCatalyticMechanismandStabilizerofAcidProteasefromAspergillusNigerS12111曹治云學(xué)科專業(yè)：研究方向：指導(dǎo)教師：申請(qǐng)學(xué)位級(jí)別：壘物絲掌墨筮壬生擅望煎生擅壘絲謝豎蛭副熬授挫騰直墼苦醫(yī)!匝堡坐亟論文提交日期：論文評(píng)測(cè)人：論文答辯日期：答辯

2、委品會(huì)主席：學(xué)位授予單位：學(xué)位授予日期：二00五年四月望建堙煎盔堂2Q壁生目ABSTRACTAcidproteaseisageneralcallforanyenzymewhichcandecomposeproteinunderconditionofaciditytAspergillusnigerandAspergillusoryzaewasthemajormoldproducingacidproteaseinindustryInorde

3、rtoexploitthecapacityofAspergitlusnigerinmolecularactingmechanismandconquertheproblemsinindustryapplication，itisofmorenecessarytoartyoutthispapeLlnthisstudy，theproductionconditionsofthreecomponentsofacidproteasefromAsper

4、gillusnigerSL21I1wereinvestigated，Majorresultswereasfollows：(I)AcidProteasewaspurifiedtoelecrophoretichomogeneitybythestepsofammoniumsulfateprecipitation，DEAESepharoseFastFlowcolumnchromatographySephac攀!S200columnchromat

5、ographyandhi曲performanceliquidchromatographyItspurityWaScheckedtobeaboutasinglebandbySDSPAGE。themolecularweightWaSabout47Kda，TheNterminalpartialsequencewasSKGSAVTTPQ，whichindicatedahi曲homologywithotherproteaseproducedbya

6、spergillus#)AcidproteaseWasnotaffectedbyPCMB，Acetylacetone，／via、CDCmodification，indicatingthatgaminogroup、arginineresidues、Mereaptoandcarboxylwerenon—essentialtotheenzymeactivityTheenzymeactivitywassignificantlydecreased

7、afterNBS，NAIandDT。rmodification，andwasslightlydecreasedafterBrAemodification。Theseresultsindicatedthatthehistidineresidues，tryptophaneresidues，tyrosineresiduesanddisulfidesgroupsseemedtobeessentialtocatalyiicactivityorlo