鮭鱒魚皮、骨低聚活性膠原多肽的制備研究.pdf

1、蘭州大學碩士學位論文鮭鱒魚皮、骨低聚活性膠原多肽的制備研究姓名：王東申請學位級別：碩士專業(yè)：植物學指導教師：蒲訓20100601Studiesonpreparationandbioactivitiesofoligopeptidesofcoilgenbyenzymaticbondinghydrolysistechnologyfromcollalenbyenzymaticbondinghydrolysistechnol02yfromTrou

2、tskinandboneABSTRACTThewholereseaehstudywasaimedatpreparativemethodofoligopeptidesofMr3000DaofcollagenwhichcanbeeasilyabsorbedbyhumanbodyandeasytoamplifybyenzymaticbondinghydrolysistechnologyfromskinandboneofcultivationT

3、routwhichliveoncoldspringUsingbiochemistryandenzymeengineeringteehnologys，processofdecolorization，Decalcification，optimizationbyquadrature，processcontrol，purificationeta1havebeendoneThecollagenpeptidesfromTroutskinhavebe

4、enextractedbyusingpapainandflavourzymemethodsTwokindsofenzymewereanalyzedbondingwithsinglefactortestandorthogonaltestmethod，atthesametimes，thewayofbleachingandprocessimprovementweretriedTheresultsshowedthattheoptimumaddi

5、—tionofpapainwastheratioofmaterialtoliquid1：22，thequantityofenzymetheO6％，temprarure60“C，pH=64，thetimeofenzymehysiswas3hours，andtheyieldofextractionofthecollagenpeptidesfromTroutskinwas1433％DH1033％Theoptimumadditionofflav

6、ourzymewastheratioofmaterialtoliquid1：20，thequantityofenzymethe035％，temprarure55℃，pH=65，thetimeofenzymehysiswas25hours，andtheyieldofextractionofthecollagenpeptidesfromTroutskinwas1368％，DH1065％Amongthreemethods，usingpapai

7、nbeforeandusingflavourzymeafter，whichisthebestmethod；ActivatedCarboncanbeusedasdecolorantFinishedproductwasallrightaftertheprocessofcolation，microfiltration，ultrafiltration，concentration，dryingandcribrationweredoneandpur

8、itycoefficientwas9104％，collagenpeptideslessthan2000Dawere8223％FARPvalue(3mg／mL)was339mmol／L，efficiencyofscavengingactivity(3mg／mL)ofhydroxyradicalwas1009％andefficiencyofscavengingactivity(3mg／mL)ofsuperoxidefreeradicalwa