用于快速檢測(cè)二噁英類(lèi)化合物的QCM生物傳感器的構(gòu)建及應(yīng)用.pdf

1、天津大學(xué)碩士學(xué)位論文用于快速檢測(cè)二噁英類(lèi)化合物的QCM生物傳感器的構(gòu)建及應(yīng)用姓名：趙勇申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：環(huán)境工程指導(dǎo)教師：劉憲華201112ABSTRACTQuartzcrysta4mierobalance(QCM，QuartzCrystalMicrobalance)sensorhasthecharacteristicsofIesponsetoabroadspectrum，highsensitivity，andsimplestru

2、cture，lowcostdetection)devices，especiallyasaquality—basedsensorsarewidelyusedinanalyticalchemisl時(shí)surfacechemistrybiochemistryandenvironmentalmonitoringandmanyotherfi61dsSurfacesensitivefilmisusedassensitivecomponentsinQC

3、MsensorsandtheA％cutquartzcrystalastransducerelementsWeusetherelationshipofquartzcrystalqlualitychangefrequency；transferthequality，concentrationsignalintofrequencyTherebyachievethepurposesofdetectingthequalityandconcentra

4、tionThepurposeofthispaperistodevelopsensorsfordetectingtraceconcentrationsAn8MHzATcutquartzcrystalresonatorwasmodifedwithreceptorandantibodyWehaveusedthattensortodetecthumanserumalbumin(HSA)and2，3，7，8TCDDintealtimeMainco

5、ntentsfindresultswereasfollows：Firstlytheprinciple，classificationandapplicationofsensorswereintroducedTheprincipleofpi—ezoelectricquartzcrystalanddetectionmethodsofdioxinwereconsideredWeintroduceanewtechnologytodetectdio

6、xinbyusingQCMSecondly，weoltainedtheexperimentalconditionstoensurethestabilityofthesystemthroughmalnyexperimentsonthetemperatureandflowrate，thoseareconstanttemperatureandthemaximumflowrateof70Itl／minFinallywetestedthesyst

7、emperformancethroughglycerinsolutionofdifferentconcentrationsthatwere5％、10％、20％、30％、40％ThirdlyselfasLsembledmonolayerwasdiscoursedTheselfassembledmonolayertechnolog【yandcharacterizationmethodsweredescribedLipoicacidwasch

8、osetoformselfa；semblymonolayerandhighinsulatingpropertiesoftheacidicthiolmonolayerhabeencharacterizedwithcyclicvoltammetryandscanningelectronicmicroscop)tFourth，abiosensorbasedonaquartzcrystalmicrobalance(QCM)wasdevelope

9、dfordynamicandrealltimedetectionofHSAThefrequencyresponsesduetotheadsorptionofthestahdardsamplesonthebiosensorsurfaceweremeasuredTheresultsshowthatHSAcanbequantitativelydetectedwiththedevelopedimmunosensorinthedoncentrat