生物酶催化合成甲苯二氨基甲酸甲酯反應(yīng)的研究.pdf

1、河北工業(yè)大學(xué)碩士學(xué)位論文生物酶催化合成甲苯二氨基甲酸甲酯反應(yīng)的研究姓名：王海鷗申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：生物化工指導(dǎo)教師：王延吉20050601竺絲墼堡些塞些!蘭三里重呈墼呈墼星壁墼塑塞THESTUDYoFTHEREACTIoNoFBIoLoGICALENZYMECATALYZlNGANDSYNTHESIZINGToLUENEDIISOCYANATEABSTRACTTolueneDiisocyanate(TDI)，animportanto

2、rganicmedium，ismainlyappliedinthemakingofpolyurethane(PU)ThemethodthatTolueneDiearbamaterrDC)issynthesizedbyDimethylCarbonate(DMClinsteadofphosgeneandthenisdecomposedandformsTDIisagreenandhealthywayBiologicalenzymeischar

3、acterizedbyastrongcatalytieactivityintheorganicsolvent，andithasmanyadvantages，suchasmildreactionrequirement，easyafterlseatmem，stablestructure，andretainingactivityetctherefore，Ichosebi0109iealenzymeasthecatalyzer，andbymea

4、nsofmathematicalanalysis，Istudied也efunctionofenzymeinthesyntheticprocessTDCbvDMCandTDAAftermakingselectionto11enzymes，papin，flavourzymc，pepsin，andeaterasefromporcineliverareprovedtohavecatalyticactivitytothereactionandIf

5、oandoutthecataiyzerthathasfairlystrongcatalyticactivityandselectivity一papainWhat’SmoreIsortedoutthemostsuitablesolventTetrahydrofuran，with15m垤papainAndanoptimizationtothereactionismade，whenthetemperatureis40℃，n(TDA)tn(DM

6、C)=t／20，andtheenzymeconcentrationis174mg／m，theTDCyieldCanreach394％afterreactingfor40minutesWiththeresponsesurfacemethodologyIstudiedtheinfluenceoftheintemctionofthreefactorstotherateofproductionofTDCThethreefactorsare：en

7、zymeconcentration(35～31_3mg／nm)，thevolumeofDMC(69~207m1)andthetemperatureforreaction(25～55℃)ThroughfurtheranalysistotheequationitisleamedthattherateofproductmnofTDChasagreatvatue402％withinchosenlimitsInladeastudytothemul

8、tiplefactorsthatwillaffecttheactivityofPapinPapinhasthebestcatalyticactivi粵underthefollowingconditions：enzymeconcentrationislmg／ml，valueofPH70，temperatureofreaction40℃，theconcentrationoftheactivatorCYSHCl004mol／1，timefor

9、reaction30minutes，andtimeforkeepingtemperature30minutesAftertheexperiment。itiSfoundthattheorganicsolventTetrahydrofuran、DMCcallinfluencetheenzymeactivitvofPapintoacertaindegree珊en也econcen拄afionofTetrahydrofuraniS60％(V～)，

10、theenzymeactiv姆aanreachitshighestvaiue12，84lO：qJ／mg；whentheconcentrationofDMCis40％(V／V)，theenzymeactivityCanreachitshighestvalue97610’U／mgKEYWORDS：toluenediisocyanate，dimethylCarbonale，toluenediearbatnate，papain，response