水牛和黃牛同種內(nèi)及種間顯微受精的初步研究.pdf

1、廣西大學(xué)碩士學(xué)位論文水牛和黃牛同種內(nèi)及種間顯微受精的初步研究姓名：牛向麗申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：石德順2003.6.1堡!：絲塞!查塑薹!墮堂塑墨登塑呈堂墨塹塑塑生塑塞一APRELIMINARYSTUDY0NTHEINTRASPECInCANDINTERSPECIFICFERTILIZATIoNBETWEENBUFFALoANDCArrLEBYINTRACYTOPLASMICSPERMINJE(_IONFac

2、torsaffectingthefertilizationofbuffaIoandbovineOOcytesbysperminjectionwereexamined，andthepossibilityofintraspecificfertilizationbetweenbuffaIoandcattlewasalsoexploredinthisstudyBUffaIoandbovineoocytesmaturedinvitrofor222

3、4hwerefertilizedbyinjectionofbuffaloOI“bovinefrozenthawedspermatozoaAt1618hafterinjection，aportionofoocyteswerefixedinaceticethanoltoexaminethepronuclearformation，otherswereculturedinvitrotoevaluatetheircleavagerateandem

4、bryonicdevelopmentalabilityTherewasnotdifierenceinthetwopronuclearformationrate(47’73％VS3667％)，cleavagerate(68％VS7263％)andblastocystdevelopment(1644％VS2239％1ofbuffaIooocytesfertilizedwithbuffaIo(intraspecies)andbovinespe

5、rm(interspecies)(p005)Thetwopronuclearformationrate(6000％)，cleavagerate(7333％)andblastocystrate(2857％)ofbovineoocytesfertilizedwithbovinesperm(intraspecies)werealsonotdifferentfromthose(4528％，7131％and3370％respectivelyp00

6、51ofbovineoocytesfertilizedwithbuffalosperm(interspecies)Treatmentofbuffalospermwith100Itg／mLlysophosphatidedidnotaffectthepercentageofbuffaIooocytescleaved(80。4l％VS8725％，p005)anddevelopedtoblastocysts(67％VS1078％，pO05)af

7、terICSISixtyfourpercentofblastocystsderivedfrombovineoocyrtesfertilizedwithbuffalospermsurvivedand306％ofthemhatchedafterfreezingthawingwhichwassimilartothehatchingrate(3478％。p005)ofblastocystsunfrozenInconclusions，1)buff

8、aIoOOcytesfertUizedwithbovinespermandbovineoocytesfertilizedwithbuffalospermcandeveloptoblastocysts，whichareprovedtobefreezable；2)thedevelopmentalcapacityofbovineoocytesishighelthanbaffalooocytesasevidencedbytheirproport