斑痣盤菌科分類及被子植物生散斑殼屬分子生物學(xué)研究.pdf

1、分類號(hào)密級(jí)單位代碼：!Q3盟學(xué)號(hào)：SQ4Q21QQ5132窟微雇業(yè)大虧學(xué)位論文斑痣盤菌科分類及被子植物生散斑殼屬分子生物學(xué)研究STUDIESONTAXoNoMYoFRHYTISMATACEAEANDMoLECULARBIoLOGYOFLDP日DDE尼M，UMoNANGIoSPERMS研究生：韭坦指導(dǎo)教師：撻墓焦麴拯合作指導(dǎo)教師：中請(qǐng)學(xué)位門類級(jí)別：理堂亟專業(yè)名稱：微生塑堂研究力‘向：微生物僉差堂所在學(xué)院：生僉抖堂堂瞳答辯委員會(huì)主席二零零七

2、年六月AbstractSomemembersofthreegenerainghytismataceaewhichcollectedfromthesouthmountainsofAnhuiprovinceweresystematicallystudiedwiththemostnoveltyandadvancedpolycharacterintegratedtaxonomymethodwhichisbasedOilthecharacters

3、ofmorphologydevelopmentalbiologyecologyanddistributionTwentyeightLophodermiumstrainsonangiospermswerecharaterizedbyRAPDPCRreactionwith9randomprimerswhosegelelectroersisdiagramscanbeamplifiedrepeatedlyandclearlyBythecompP

4、AisonofRAPDanalyticresultsandthephenotypiccharactersindicatedtheimportanceofsomemorphologicalcharacterssuchastheembeddedpositionofascomatastromamasci，asenspores，paraphysesandthecategoryofhosts0ntheclaSsificationofspecies

5、levelRelationshipsofsomeintraspecificandinterspecificSl雕linSofLophodermuimonangiospermswereaffirmedThemainresultisasfollows：(1)Nearly300specimensfromthesouthmountainsofAnhuiprovinceandAnhuiAgriculturalUniversityForestPro

6、tection(AUUFP)wereidentifiedaccordingtothetaxonoicprinciplesofDarker(1967)Cannon&Minter(1986)andKirketa1(2001)，8speciesofRhytismataceaewerestudied，includingCLimitatusofCoccomyces，HstephanandraeofHypoderma，Lagathidis，三imp

7、licatum，三minus，Lrufum，LmenispermiandLphotiniaeofLophoedemium1tstephanandraeLmenispermiand厶photiniaearenewspeciesamongthemDichotomouskeytOthesespeciesWerecompliedMorphologicalcharacteristicsweredescribedandillusteated，and

8、habits，hostsanddistributionswrerecordedforthespecies(2)LivingfungimaterialscollectedfromtheNationalforestparkofTiantangzhai，AnhuiwereisolatedinMAculturemediabytissueisolationtechnics，and9Lophodermiumstrainsonangiospermsw

9、ereobtainedDuringtheperiodofthesinglepointcultivatingculturecharacterofthesamespecies’fungiweredistinct，eventhesamespeciesofdifferenthostsweredifferentingrowthratecolonysizecolonycolorandpigmentproducingEachisolatedstrai

10、nsattachesthedescriptionoftheculturecharacter(3)9primerswhichiscomposedof10oligoribonucleotideareusedtoanalyzethegeneticdiversityof28Lophodermiumstrainsonangiosperms105lociaredecetedintotalamongwhichthereare100polymorphi

11、clociThepercentageofpolymorphiclociis9524％DendrogramofgeneticrelationshipsamongLophodermiumstrainsonangiospermsisconstructedontheUPMGATheresultdemonstratesthat28Lophodermuimstrainsonangiospermsareclusteredinto7groupsinge