蜜環(huán)菌優(yōu)良菌株的篩選、鑒定及對(duì)烏天麻產(chǎn)量的影響.pdf

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文蜜環(huán)菌優(yōu)良菌株的篩選、鑒定及對(duì)烏天麻產(chǎn)量的影響姓名：季寧申請(qǐng)學(xué)位級(jí)別：碩士專(zhuān)業(yè)：植物病理學(xué)指導(dǎo)教師：李玉20080601AnOptimizedScreening，IdentificationofArmillariamelleacomplexandInfluenceonYieldofGastrodiaelatafglaucaAbstractArmillariamelleacomplexisallimportantw

2、orldwidepathogen，whichhaswidehostsandstrongpathogenticabilityAmelleacomplexhasmanyuniquecharacteristics，suchasitisparasitismandsaprophytism，alsosymbioticwithGastradiaelataandGrifofaumbellateAmelleacomplexisoneofthemedici

3、nalandediblemushroomswithhiigheconomicvalueGelataispreciousmedicinalplantwhichhastheabilityofcuringchildreninfantileconvulsion，apoplexyandneuralgiaFurthermore，recentstudiesshowedthatithadsomecurativeeffectonseniledementi

4、aGelataisaveryunusualplantIthasnorootnogreenleafSOitCan’tbephotosyntheticandabsorbnutrientsAmelleacomplexisabsolutelynecessaryduringthegrowthofGelata，whichprovidesmostnutrientsSoitisimportantforincreasingyieldandqualityt

5、ocultureoptimizedArmillariastrainsGelata￡elataandGelatafglaucaaretwomainvarietiesinGelatacultivationofJilinProvinceatpresentGelatatgl鋤cnisofgoodmorphology，excellentqualityandhigherdryingrate，whichhasconsiderableeconomicv

6、alueSofourteenArmillariastrainswereobtainedbyisolatingandculturingfromwildArmillariafruitbodiesandGelatacollectedinmainGelataproductionareasofChangbaiMoutainofJilinFiveoptimizedArmillariastrainswerescreenedtocultivatewit

7、hGelata￡g／口ucaFinallyoptimizedstrainsweredeterminedandbiologicalspecieswereidentifiedBelowworkswerechieflycompleted，andcorrelatedconclusionswereobtained1ScreeningofculturemediaforAmelleacomplex。Growthcharacteristicsofthe

8、At1lstrainondifferentculturemediawereinvestigated，andmyceliabiomassatdifferentperiodsweremeasuredTheresultsshowedthatrhizomorphoftheAr11strainonthepeptoneculturemediumgerminatedfastanddevelopedstrongly，shortfullborictime

9、，andhighermyceliabiomassweremeasuredSothepeptoneculturemediumWasscreenedforculturingtheArmillariastrainsfastHighermyceliabiomasswasmeasuredwhentheAr1lstrainculturedonthePDAculturemediumat25℃onthe25thdayItshowedthattheAr1

10、1strainculturedonthePDAculturemediumgrewstablyanddelayedsenescenceSoitWasusedtopreservetheArmillariastrains2TheoptimizedArmillariastrainswerescreenedGrowthcharacteristicsofthefourteenArmillariastrainsculturedonsolidandli

11、quidculturemediawerestudiedThefiveoptimizedstrains(Ar一04、Ar07、Ar11、Ar13、At14)werepreliminarilyscreened，whichwereusedforGelatacultivationThecharacteristicsofthefiveoptimizedstrainswerefurtherdetermined3Thecultivationexper

12、imentWasmadetostudytheeffectofdifferentArmillariastrainsontheyieldofGelatatheAr04、Ar11strainswerescreenedcomparedtothecontrolbetweenbionicswildcultivationandoutdoorboxplantingTheconclusionshowedthattheAr04strainexhibited