高產(chǎn)木聚糖酶芽孢桿菌的分離篩選及其酶性質(zhì)研究.pdf

1、四川農(nóng)業(yè)大學(xué)碩士學(xué)位論文高產(chǎn)木聚糖酶芽孢桿菌的分離篩選及其酶性質(zhì)研究秦玉花指導(dǎo)教師倪堂勤副熬攫學(xué)科專業(yè)邀生物堂研究方向弛物微生盔2006年5月SelectionofBacillussppOverproducingXylanaseandStudiesonEnzymicPropertiesSichuanAgriculturalUniversityYa’anSichuanChina625014AbstractXylanaseproducing

2、strains(Bacillusspp)wereisolatedfromruminalcontentsinbuffaloandgoatIsolatesofBacillussppwereobtainedandassayedforthexylanaseactivitywithamethodoftransparentzonesL2—19andY25strainspresentedthehighestactivitywereselectedfo

3、rfurtherinvestigationindetailAccordingtothemorphologicalandphysiologicalcharacteristics，twostrainswereidentifiedasBacillspumilusandBacillussubtlTisrespectivelyBasedonthestudyofxylanase，theextractingmethodsandconditionsof

4、xylanfromCOrncobwereimprovedThevirtueofthecookingmethodwasintegratedwiththeaIkaliextractingmethodThemainprocessofextractingconsistsofcooking，alkalinesoak，anddepositingThecontentofhemicelluloseinthecrudexylanproductis634％

5、andtheratioofextractingis276％ThecultureconditionsthatCaninfluencethegrowthandenzymeproductionwereinvestigatedThebestconditionforL2—19producexylanasewerewheatbranusedascarbonandyeastasnitrogensourceWhenwheatbranwasusedasc

6、arbonand(NH4)2SOaasnitrogensource，thexylanaseproductionofY25reachedthemaximalBoththestrainsreachmaximumactivityatintitialpH60andenoughoxygenThecrudexylanaseofL2—19strainkeptmaximallyactiveatpH70and50“C，bestableatpH6O；and