產(chǎn)甲烷細(xì)菌的分離及其生理學(xué)特性的初步研究.pdf

1、貴州大學(xué)碩士學(xué)位論文產(chǎn)甲烷細(xì)菌的分離及其生理學(xué)特性的初步研究姓名：張萬芹申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：康冀川20080501★貴州大學(xué)2008屆碩士學(xué)位論文★AbstractMethanogensisaspecialprokaryoticorganismwhichproducelargequantitiesofbiogasastheendmumberthatexistinwLriousextremistanaerobicen

2、vironmentstoproduceenergyAsthefinalmemberofanaerobicbiologicaltreatmentinnatllralc翹Jooncycle，itwansformlargenumberoorganicmatterintenewableenergywithotherflorabacteriassynergyItplayimportantroleinpromotingthecycleofthena

3、ttl托materialandsolutionfortheenergycrisisMethanogensistheCOreofmethanefermentationdevelopmentthebiogaswhichisthekeytosolutiontheruralenergy，Sothecontentsofmenthanogensinthefiredamppoolandthegrowthdirectlyimpactonthequali

4、tyofgasThisstudysimplysummariedthebiomassandbiogasfermentationwhichcloselyrelatedwithmethanogens，andthemethanogens’roleinmethanefermentation(anaerobicdigestion)、probablysummariedit’Sphysiologicalcharacteristicsandecologi

5、calcharacteristics、it’sresearchprogressandresearchsignificanceAccordingtothemethanogens’Smetabolismandthegrowthcharacteristicsintheenvironmentthestudyoptimizethemediumandthespecificseparationmethods，obtainedthemediumandt

6、heseparationmcthodswhicharesuitableformethanogens’SgrowthTheculturemediumwithamixtureofchemicalsWaStestedfortheisolationofmethanogenicbacteriaAboutthemethodsThefirsttimetousethesmallinvertedglasstocollecttllegaswhichispr

7、ocessedbymethanogens，andasthebasistodeterminewhetherthereisanymethanogengrowth，thefirsttimetoanalysethemembolitesiag麒licatsThisstudyobtainedfivepuremethanogenicbacteriasbyusedtheoptimalmediumandseparationmethodaccordingt

8、otheirmorphologicalandbiochemicalcharacteristics，Initiallyidentifiedastheacidm礎(chǔ)anebacillispAndfirsttoanalysethecompositionofthemetabolites，obtainedmethanogenicbacteria’scontentofthemethanewhichismorethan98％Accordingtothe

9、growthofit’sfean鵬，thisstudyreportedthemethanogenicbacteria’SpreservationmethodsInthisstudyfivemethanogens’growthmatrix、PHvalue，temperature，tolerancethesaltconcentrationandpenicillinconcentrationwarestudiedfivestringmetha

10、noheusarcusedonlyacidsodiumforgrouth，theyoptimumgrowthrangeofPHvalueare60t080，Theoptimumgrowthtemperatureis37℃thetolerancetothelargestsaltconcentrationof2％t04％，Andthemostresistanttopenicillinconcentrationis128t0256millio