絲石竹皂甙對人胃癌細胞SGC-7901增殖、凋亡的影響及機制研究.pdf

1、分類號：R735.2密級：非密UDCUDC：610學校代碼：11065碩士學位論文絲石竹皂甙對人胃癌細胞絲石竹皂甙對人胃癌細胞SGCSGC79017901增殖、凋亡的影響及機制研究增殖、凋亡的影響及機制研究辛超指導教師岳麓副教授學科專業(yè)名稱腫瘤學論文提交日期2016年4月19日論文答辯日期2016年5月31日答辯委員會主席Gypsophilasaponinsinhibitproliferationpromoteapoptosisofga

2、striccancercellsthroughdownregulationofWntgenesexpressionAbstractAIM：ToinvestigatetheeffectofGypsophilasaponinsonhumangastriccancerSGC7901cellsitsmechanism.METHODS：SGC7901cellswerechosenasthestudyobject.Theexperimentalgr

3、oupwastreatedwithGypsophilasaponinsofdifferentconcentrations（204080ugml）f24、4872h.ThecontrolgrouponlycontainsthesamevolumeofculturemediumwithoutGypsophilasaponins.ThecellviabilitywasmeasuredbyMTTassayFlowcytometrycombine

4、dwithAnnexinVPIdoublestainingmethodwasusedtoanalyzecellapoptosiscellcycleQuantitativeRTPCRwasusedtodetecttheexpressionsofWnt5aWnt8bgenesaftertreatingwithGypsophilasaponinsofdifferentconcentrationsf48h.RESULTS：（1）①Thecell

5、survivalratebetweendifferentgroupswhichweretreatedwithGypsophilasaponinsofdifferentconcentrationsfthesametimeshowedstatisticallysignificantdifferences.Thecellsurvivalratesof24h:Thecontrolgroupwas100.00%theexperimentalgro

6、upwas（82.821.64）%、（50.581.04）%（20.000.75）%respectively.F=846.157，p0.01.Thecellsurvivalratesof48h:Thecontrolgroupwas100.00%theexperimentalgroupwas（76.651.48）%、（40.281.40）%（16.380.88）%respectively.F=896.265，p0.01.Thecellsu

7、rvivalratesof72h:Thecontrolgroupwas100.00%theexperimentalgroupwas（70.571.60）%、（27.471.21）%（10.090.45）%respectively.F=957.040，p0.01.②Thedifferencesofthecellsurvivalratebetweendifferentgroups(exceptthecontrolgroup)whichwer

8、etreatedwithGypsophilasaponinsofthesameconcentrationfdifferenttimewerestatisticallysignificant.Whentheconcentrationis20ugmlthecellsurvivalrateofexperimentalgroupswereanalyzedbyonewayANOVA:F=45.281p0.01.Whentheconcentrati

9、onis40ugmlthecellsurvivalrateofexperimentalgroupswereanalyzedbyonewayANOVA:F=217.583p0.01.Whentheconcentrationis80ugmlthecellsurvivalrateofexperimentalgroupswereanalyzedbyonewayANOVA:F=134.698.p0.01.③TheIC50valueof24hwas

10、41.171gmlwhichwascalculatedbystatisticalsoftware.（2）Thecellapoptosisrateof24h:Thecontrolgroupwas(15.110.95)%theexperimentalgroupwas(20.330.78)%、(23.931.18)%(27.671.44)%respectively.F=89.621，p0.01.（3）Cellcyclewasanalysedb

11、yflowcytometryaftertreatingwithGypsophilasaponinsofdifferentconcentrationsf24h.TheG0G1period:Thecontrolgroupwas（53.991.35）%theexperimentalgroupwas（58.401.77）%、（62.400.85）%（65.431.18）respectively.F=42.792，p0.01Speriod:The