Pt1a期腎透明細(xì)胞癌患者癌中心灶、癌旁及正常腎組織的蛋白質(zhì)組學(xué)研究.pdf

1、青島大學(xué)碩士學(xué)位論文Pt1a期腎透明細(xì)胞癌患者癌中心灶、癌旁及正常腎組織的蛋白質(zhì)組學(xué)研究姓名：王永強(qiáng)申請學(xué)位級別：碩士專業(yè)：外科學(xué)（泌尿外科）指導(dǎo)教師：劉勇20080531Proteomicanalysisofcancercentercancel=adjacentandnormalkidneytissueforPTlarenalclearcellcarcinomaAbstraetObjective：Toanalysisthediffer

2、encesofproteinexpressionofrenaltumortissue，canceradjacentandnormaltissueandtoconfirmtheoptimalsurgicalmargirlMethods：Byusingsurfaceenhancedlaserdesorptionionizationtimeofflightmassspectrometry(SELDITOF—MS)toanalysisfourc

3、aseswithPTlarenalclearcellcarcinomalesionsofthecancercentercanceradjacent0to05cm，canceradjacent05to1cmandnormalrenaltissue，tesearchthedifferencesinproteinexpressionResults：Threedifferentproteinpeaksdetectedweremolecularw

4、eight(MW)137439Da、139425Daand169527DainCancerCenterandnormalrenaltissuelesionsitsproteincontentweresignificantlydifferent儼OOO1)TheproteinMW137439Da、139425Dainthecancercenterandadjacent0to05cmhavehighexpressionincontrastt

5、o喇a(chǎn)cent05tolcmandnormalkidneytissuewithoutexpres西on(P0001)Otherwise，theproteinMw169527Dainnormalkidneytissueandadjacent05to1cmhavehighexpression，bycontrast，thecancercenterand鋤acent0to05cmnoexpression(尸0001)Conclusion：The

6、remaybetumorinfiltrationorresiduesinadjacent0to05cm，butnotexistinadjacent05tolcmSurgicalmarginshouldbelcmfarfromtheouteredgeoftumor，atleast05cm，totrytoavoidusingtumorenucleationPostgraduatestudent：YongqiangWang(Urology)D