中醫(yī)不同治則代表方藥對胃癌細胞端粒酶活性及細胞凋亡的影響.pdf

1、分類號密紐：單位代碼學號10441山r虧中醫(yī)藹天虧碩士學位論文論文題目中醫(yī)不同治則代表方藥對胃癌細胞端粒酶活性及細胞凋亡的影響@研究生姓名入學年月學科專業(yè)指導教9ip學位類型李昕2003年9月中西醫(yī)結(jié)合基礎(chǔ)聶克醫(yī)學科學學位2006年4月20日EmctofTraditionalChineseMedicineonBGCceH’sTelome豫seActivityandApoptosisSpeciality：BasicResearchofIn

2、tegratedTCMandWesternMedicineAuthorLiXinTutor：NieKeAbstractobjegtlve：Cancerisamajordiseasewhichaffectspeople’shealthandlifeseverelyTheTraditionalChineseMedicinehasaninimitableeffectontumorOneoftheTraditionalChineseMedici

3、ne’scharacteristicisdiagnosisandtreatmentbasedontheoverallanalysisofsymptomsandsigns，whichmeansherbalistdoctorsmaychoosequitedifferenttherapeuticprinciplesandthehomologousprescriptionsbasedondifferentsymptomsandsignsdesp

4、itethesametumorAnd，theyusuallygainthewellpleasingeffectBut，sofarthemechanismhasn’tbeenclarifiedInordertoexplorethemechanism，thisstudyresearchedseveralprescriptionsbyusingFlowCytometerandSerumPharmacologyMaterialsandMetho

5、ds：WistarratsweredividedrandomlyintoeightgroupsTheratsineachgroupweretookdifferentdecoctionstopreparepharmicratserumThentheBGC—cellswereculturedinusingtheratSelalmNextindexessuchasMTrapoptosisandtelomeraseactivityweretes

6、tedandanalysedResults：(1)Compared、vitllnormalgroup，pharmicserumgroupsshowedsignificantinhibitorygrowthofBGCcellsAndtheinhibitionisremarkablyincreasedasweincreasetheconcentrationorprolongthetime(2)Therewassignificantincre

7、aseofapoptosisrateasweaffiliatethepharmicserumtothecells’system(3)ThelevelsoftelomeraseactivitywereloweraftertheBGCcellswereculturedinpharmieserumConclusions：pharmicserumgroupsshowedsignificantdiferrenceintheareaofinhibi