萘二酰亞胺新型核酸探針光化學(xué)性質(zhì)研究.pdf

1、同濟(jì)大學(xué)碩士學(xué)位論文萘二酰亞胺新型核酸探針光化學(xué)性質(zhì)研究姓名：鄭娟申請學(xué)位級別：碩士專業(yè)：有機(jī)化學(xué)指導(dǎo)教師：江致勤20050301AbstractABSTRACTInthisthesis也ephotochemicalfeaturesofthreenovelNSubstituted18一naphthalimidefluorescenceprobesofnucleicacidsandtheirprecursorsWerestudied1Ti

2、meresolvednanosecondlaserflashphotolysistechniquewasutilizedtoinvestigatethetransientabsorptionspectroscopyofthefluorescenceprobes，18naphthalirnide—N—aceaticacid(Ni2)and18naphthatimideNcaproicacid(Nt3)。Thetransientinterm

3、ediatesofN12andN13weredetectedThetransientabsorptionspectraofNl2andN13inphosphatebuffersolution(pH270)uponpulseexcitationat355nmdisplaytwobandsat390nrnand480nlRrespectivelyulongwithashoulderaround460nlnThetripletstateWas

4、provedastheprimaryexchedstate’N12and’N13underwentelectrontransfer(ET)、Ⅳimtriphenylamine(TPA)toproduceN12一(orN13一)andTPA“ItWasfoundthatintheabsenceofquenchers，theselfiquenchingoftriplets婦sbythegroundstatesofN12OrN13tookpl

5、aceviaelectrontransferprocessPhotoinduccdETmechanismwassuggestedandselfquenchingrateconstantwasfurtherdeterminedThephotoinducedETfromnucleosides，guanosine(GUA)and2deoxyguanosine(DGUA)to’N12or’N13wasalsoexploredbyusinglas

6、erflashphotolysisBothN12andN13showedastrongelectronacceptingabilityMoreovertheformationoftheradicalanionofN12andN13isfacilitatedinagreatextentsimultaneouslyThebimolecularquenchingconstantsofN12orN13byGUAandDGUAweredeterm

7、inedTheelectrontransferprocessbetweenN12OrN13andnucleosidesWasverifiedandtheETmechanismWasproposed2FluorescencequenchingspectroscopytechniqueWaSusedtoinvestigatethestaticfluorescencequenchingbetweenNI(includingN(2’hydrox

8、yethyl)1，8一naphthalimide(N11)，N12andN13)andnucleicacidprecursorTheresultsshowthattherearestrongfluorescencequenchinginteractionsTheapparentaffinitybindingconstanthasbeendeterminedUVspectroscopyandfluorescenceofsingleprob