工業(yè)化提取分離純化獨(dú)一味總環(huán)烯醚萜苷類成分

1、工業(yè)化提取分離純化獨(dú)一味總環(huán)烯醚萜苷類成分工業(yè)化提取分離純化獨(dú)一味總環(huán)烯醚萜苷類成分邱建國，張泉龍，尉麗力，李茂星，賈正平，張汝學(xué)，樊鵬程，周珺（蘭州軍區(qū)蘭州總醫(yī)院藥材科，甘肅，蘭州730050）[摘要]目的目的應(yīng)用大孔吸附樹脂結(jié)合聚酰胺柱色譜法富集分離獨(dú)一味中總黃酮、總環(huán)烯醚萜苷及大極性成分實(shí)現(xiàn)工業(yè)化生產(chǎn)。方法方法用CCPP系列層析設(shè)備進(jìn)行分離富集，一階導(dǎo)數(shù)紫外分光光度法測定總環(huán)烯醚萜苷含量、高效液相色譜法測定山梔苷甲酯，8O乙酰山梔

2、苷甲酯并考察不同洗脫條件下有效成分總環(huán)烯醚萜苷的變化。結(jié)果結(jié)果采用70％乙醇溶液為溶媒。大孔吸附樹脂柱用“下進(jìn)上出”的進(jìn)樣方式和“上進(jìn)下出”的洗脫方式時(shí)，得率為14.84％，山梔苷甲酯的轉(zhuǎn)移率為33.76％，8－O－乙酰山梔苷甲酯的轉(zhuǎn)移率為32.99％。結(jié)論結(jié)論此生產(chǎn)工藝方法及參數(shù)可以成功地將實(shí)驗(yàn)成果轉(zhuǎn)化為工業(yè)化生產(chǎn)。[關(guān)鍵詞關(guān)鍵詞]獨(dú)一味；總環(huán)烯醚萜苷；分離富集；工業(yè)化生產(chǎn)[中圖分類號中圖分類號]R286TQ460.6[文獻(xiàn)標(biāo)識碼文獻(xiàn)

3、標(biāo)識碼]A?ExtractionPurificationofTotalIridoidGlycosidesinHerbaLamiophlomisRotatainIndustrializationQIUJianguoZHANGQuanlongWEILiliLIMaoxingJIAZhengpingZHANGRuxueFANPengcheng，ZHOUJun(DepartmentofPharmacyLanzhouGeneralHospital

4、ofPLALanzhou730050China)Abstract:ObjectiveThemacropousadsptiveresinsvhromatotraphiccolumncombiningwithpolyaechromatographiccolumwasusedtoseparatetheflavonoidesiridoidglycosidesmaximuspolarityingredientsinindustrializatio

5、n.MethodsTheflavonoidesiridoidglycosidesmaximuspolarityingredientswereseparatedenrichedbyCCPPseriallaminaranalysisequipmentcontentoftotaliridoidglycosidesingredientswasassaiedbythefirstderivativespectrophotometrycontento

6、fshanzhisidemethylester8oacetylshanzhisidemethylesterweredeterminedbyHPLCchangeoftotaliridoidglycosidesingredientswasinvestigatedindifferentelutionrequirement.Results70%Alcoholwasusedasdissolvant.Usingthe“introductionfro

7、minferisurface”sampleintroductionmode“introductionfromsuperisurface”elutingmodeinthemacropousadsptiveresinsyieldwas14.84%transfusionratiofshanzhisidemethylester8oacetylshanzhisidemethylesterwas33.76%32.99%respectively.Co

8、nclusionThisproductiontechnologymaytransfmexperimentalachievementtoindustrialproductionsuccessfully.[Keywds]HerbaLamiophlomisRotata，TotalIridoidGlycosides，separatingenriching，industrialproduction本課題組通過藥理實(shí)驗(yàn)篩選，首次確定了獨(dú)一味總環(huán)烯醚

9、萜苷是獨(dú)一味的止血、鎮(zhèn)痛活性部位，?[收稿日期][通訊作者]★賈正平，主任藥師，教授，博士生導(dǎo)師，主要從事醫(yī)院藥學(xué)專業(yè)的研究。Tel：0931－8994652；Email：jiazp166@圖1工業(yè)化分離、純化獨(dú)一味總環(huán)烯醚萜苷成分示意圖Fig.1ConceptualdiagramofextractionpurificationofTotalIridoidGlycosidesinHabraLamiophlomisRotatainIndu

10、strialization裝柱及其前處理：裝柱及其前處理：將每根聚酰胺柱裝入聚酰胺粉40kg，大孔吸附樹脂柱100kg大孔吸附樹脂裝入中，常水沖洗至大孔吸附樹進(jìn)樣速度：進(jìn)樣速度：≤100Lh1進(jìn)樣方式：進(jìn)樣方式：聚酰胺柱，上進(jìn)下出；大孔吸附樹脂柱，下進(jìn)上出；即打開聚酰胺柱的“上進(jìn)”、“上排”、“進(jìn)料”、“串出”閥門，大孔吸附樹脂柱的“下進(jìn)”、“串進(jìn)”、“上排”、“出料口”閥門，聚酰胺柱的“上排”管流出溶液時(shí)，關(guān)閉此閥門，大孔吸附樹脂柱的