氣相色譜-質(zhì)譜聯(lián)用法測定廣玉蘭果殼和種子中揮發(fā)性化學(xué)成分(已發(fā)表)

1、1氣相色譜氣相色譜質(zhì)譜聯(lián)用法測定廣玉蘭果殼和種子中揮發(fā)性化學(xué)成分質(zhì)譜聯(lián)用法測定廣玉蘭果殼和種子中揮發(fā)性化學(xué)成分范小春1，2，梁光義1，2，曹佩雪2，王道平21貴陽中醫(yī)學(xué)院，貴陽550002；2貴州省、中國科學(xué)院天然產(chǎn)物化學(xué)重點(diǎn)實(shí)驗(yàn)室，貴陽550002摘要摘要：目的目的:分析廣玉蘭果殼和種子的揮發(fā)性成分，并比較其異同與含量差異。方法：方法：采用水蒸氣蒸餾法分別提取果殼和種子中的揮發(fā)性成分，用氣相色譜質(zhì)譜對(duì)其揮發(fā)性成分進(jìn)行鑒定。結(jié)果結(jié)果：從

2、廣玉蘭果殼中分離出111種組分，共鑒定了79種化合物，占揮發(fā)油總量的56.54%；從種子中分離出88種組分，共鑒定了58種化合物，占揮發(fā)油總量的46.02%；其中果殼和種子的共有成分21種（果殼占總揮發(fā)油含量的28.46%，種子占33.77%）。結(jié)論：結(jié)論：為進(jìn)一步研究開發(fā)廣玉蘭的果殼與種子提供依據(jù)。關(guān)鍵詞關(guān)鍵詞：廣玉蘭；揮發(fā)性成分；氣質(zhì)聯(lián)用（GCMS）AnalysisofVolatileChemicalConstituentsinHu

3、llSeedsofMagnoliagriflabyGCMSSpectrometryFANXiaochun12LIANGGuangyi12CAOPeixueWANGDaoping（1.GuiyangCollegeofTraditionalChineseMedicine，Guiyang550002，China2.ThekeylabatyofChemistryfNaturalProductsofGuizhouProvinceChineseAc

4、ademyofSciencesGuizhouGuiyang550002）ABSTRACT：OBJECTIVE:ToanalysecomparethevolatilecomponentswhichwereextractedfromhullseedofMagnoliagrifla.METHODS:ThevolatilecomponentswereextractedfromhullseedsofMagnoliagriflabysteamdis

5、tillationthechemicalcomponentsofthemwereidentifiedbyGCMS.RESULTS:Onehundredelevencompoundswereextractedfromthehullseventyeightcompoundswereidentifiedaccountingf56.54%ofthetotalvolatileoileightyninecompoundswereextractedf

6、romseedfiftyeightcompoundswereidentifiedaccountingf46.02%ofthetotalvolatileoil.Twentyonecompoundswerecommoninbothofhullseeds.CONCLUSION:TheresultsofthestudiescanbereferencedwhenthehullseedsofMagnoliagriflaneedtobefurther

7、studied.Keywds：Magnoliagrifla；VolatileChemicalConstituents；GCMS廣玉蘭（Magnoliagrifla）為木蘭科木蘭屬植物，又稱大花玉蘭、洋玉蘭、玉蘭，主產(chǎn)地為我國長江流域以南地帶。廣玉蘭葉、花、種子、果殼均可入藥，其葉及花中含有揮發(fā)油、木蘭花堿等多種化學(xué)成分，果實(shí)中含有脂肪酸，花、葉可提取香料，種子可榨油，其葉乙酸乙酯提取物中還含有黃酮類和酚類化合物，葉和果實(shí)中含有五種倍半萜

8、內(nèi)酯[12]。其幼果水提取物含生物堿、皂苷及黃酮苷，乙醇提取物含黃酮和強(qiáng)心苷[34]。文獻(xiàn)研究未發(fā)現(xiàn)有對(duì)廣玉蘭果殼和種子中揮發(fā)性成分進(jìn)行對(duì)比性研究。本文采用水基金項(xiàng)目：金項(xiàng)目：國家中醫(yī)藥管理局重點(diǎn)學(xué)科，黔科合計(jì)工字[2009]4008號(hào)作者簡介：作者簡介：范小春，女，碩士研究生，研究方向：主要從事天然藥物化學(xué)研究。Tel：（0851）3807713Email:409271850@通訊作者：梁光義，男，碩士學(xué)位，教授，博士生導(dǎo)師，研究方向

9、：主要從事天然藥物化學(xué)研究。Tel:08515652109Email:310.0015.0020.0025.0030.0035.0040.0045.0050.0055.002000004000006000008000001000000120000014000001600000180000020000002200000240000026000002800000TimeAbundanceTIC:9102210.D圖1廣玉蘭果殼的揮發(fā)性成分總

10、離子流圖廣玉蘭果殼的揮發(fā)性成分總離子流圖10.0015.0020.0025.0030.0035.0040.0045.0050.0055.0060.002000004000006000008000001000000120000014000001600000180000020000002200000240000026000002800000300000032000003400000360000038000004000000TimeAbund

11、anceTIC:9102211.D圖2廣玉蘭種子的揮發(fā)油總離子流圖廣玉蘭種子的揮發(fā)油總離子流圖表１表１廣玉蘭果殼與種子中揮發(fā)油化學(xué)成分分析結(jié)果廣玉蘭果殼與種子中揮發(fā)油化學(xué)成分分析結(jié)果相對(duì)含量(%)序號(hào)化合物分子式果殼種子1pCymenep傘花烴C10H140.0500.1122Chavicol佳味醇C9H10O0.0340.0873αCubebeneα庫比烯C15H240.0972.7094αBergamoteneα香柑油烯C15H24

12、0.1000.1295βCaryophyllenβ石竹烯C15H242.6304.3856GermacreneD大櫳牛兒烯DC15H241.6451.568737Guaiadiene37愈創(chuàng)木二烯C15H240.1330.1718αAmphene()α紫穗槐烯C15H243.4916.2109βSelineneβ芹子烯C15H243.8460.38310δCadineneδ桅子C15H246.41513.65511()Aromadend