銀杏、厚樸內(nèi)生真菌的初步研究.pdf

1、成都中醫(yī)藥大學(xué)碩士學(xué)位論文銀杏、厚樸內(nèi)生真菌的初步研究姓名：龐蕾申請學(xué)位級別：碩士專業(yè)：生藥學(xué)指導(dǎo)教師：嚴(yán)鑄云20050401成都中醫(yī)藥太學(xué)2002級碩士學(xué)位論文ResearchontheEndophytesin6婦置印6洳64Land，ll磊g抖口婦幼‘lc跏口施RehdetWilsM|jor：Ph_macognocyMaster’sD％mCandI哇ate：Pn窖kjMentor：Y曩H軸uyunAbstractEndophytes

2、a∞他fe玳泔to如ngjcolonizingh％lfhypl明ttis5ucswithoutcausingovertsymptomsinor印parcm州uryt0thehostRes％rchresullsjnne鰣ye盯sshowt11atendophytecanproducesomemetaboIitessimilartotllosefbmtllehostpI蚰tThisreaturecouldmm∞endoph”ebecarne

3、anewresourcewhichc帆啪ducesomecompoul以swithnovds打uctu∞張dspeclncbioactivitytF甜《bove糟鰱onsweselcctedGf玎婦D6如施L肌d脅哪D婦西ici竹口凰Rehdet啪】sasolIrori窟inaIm砒erials，toisoIateandidenti臼theirendophyticfimgi，肌dsinoutthebbactiveS仃ai船f如mtIem

4、Me蛐while，westudiedthebioactivitiesofonebioactives婦ininintialsta留eAccordingt0ourres腳∽hfindings，weconcludedtllcabovecontentsasfolIowin疊：Firstly，178strainsendophyteswe磚is01atedffomtherootsandstemsofGj一幻靜6洳6ⅡLandtheyweremorp

5、hoIogi∞IIyidentifiedbelongingto28gcn盯8’6加nilies，4orders8ndlclassMoniIiales，whichiIlcludes966％oft】1ewholes”alnsisbelievedtlledominantordeLWhiIe，如口一“m，爿印刪脅峨a口如spD一”marebelicvedthedominantgenera，which腿yinclude365％，19，7％，lO1

6、％oft1cwhoIesn甚insinmspectiveItisshownth砒quantity’speciesanddislributifl窖Oftheendophyt沁內(nèi)ngla心variedindifrercnto嚼啪sof鋤^j筘6洳6口LMe帥while，weus酣TLCmemodino—ertocomp甜etheme倒boIit船of178s打8inswithterpenoidsIactone舭dtlle他sultshows

7、tIatthemetaboIitesof36s打ainsmayincludGtcrpenoidslactoneOn廿Icomerhand，tllemsultofinhbitiohagainstplgteleta罩溶旭g哦ingexperimentproV髂t11at7stminsmayproduceginkgolideCorsimiIarcompO鄴dSjSecondly48strajnsendophyteswe陀isolatedf沁m

8、mestcmsofAf卿即D婦夠c加口，fsRchdetWiIsAsa心su也tlleyweremorphologicallyidem譎edbeIongingto16窯enefa，6血milies，3orders粕dlcl舔sM0niJiales，whjchincludes333％oft11ewhoIe8t忸ins，isbelievedtlledomin硼1tordeLWhile一印e憎塒?？mfcf脅“卅，F(xiàn)如口一“marebeli

9、evedthedominantgenem，wh沁h(yuǎn)include313％，188％，146％oftllewholestminsseparatelyItIsalsoshowntllatqu粕ti饑species撲ddi蛐rIbutingoftIleend0曲ytic如n窯iarevBriedindi脅enth曲itatof肘婦^o婦瑚BdH口脅RehdnWIIs1IheresultofmeHPLCexperimenttometabotes

10、of27sn丑insindic砒cstIIemyceliumorferm朋tedliquidof17strainsmayincludehonokiol。甲a|陰ololorsimiI盯compoundsInaddition，tlIeresultof蚴tbacterlal8ssayshowsthat7strainsoftlIe如ngihaveantagonismagainstoneormorebacterja’such船&印J砂，Dcoc

11、c螂口“，螂，j強(qiáng)c^P—c^抽cD“。只卵u面m橢甜aen理伽∞dAfI盯球peale羽ysined，weselectedFM044，whichhadbetterantibactcriaIe矗bcLt0studyantibacterialcompoundsBythisprocess，ac叫stalw夠i∞l觚edfromthemyceliumofFM044們dbst鄺cturew夠identmed姻Dm蚴nitolbysomes打uc