血液細(xì)胞分析儀原理及應(yīng)用

1、Principle and application of blood cell analyzer (血液細(xì)胞分析儀原理及應(yīng)用),,Human physiological and pathological changes, often caused by changes in blood components, so that a timely understanding of the changes in the blood compo

2、nent, can provide a diagnosis and treatment of diseases of the important basis for doctors.,,Red blood cellBiconcave disc SeedlessRed blood cell diameter between 6 ~ 9 m, an average of 7.2 M.The number of red blood ce

3、lls of the adult male was (4.6 ~ 6.2) * 1012 /LThe number of red blood cells of the adult female was (5.4 ~ 4.2) * 1012 /L,,White blood cellNuclear sphereThe diameter is 7 ~ 25 M.The number of white blood cells in no

4、rmal persons was (10 ~ 4) * 109 /L,,PlateletBoth sides of the micro concave disk, when stimulated pseudopodiaThe normal platelet value (100 ~ 300) * 109 /L, diameter 2 ~ 4 µm.,,Hemoglobin (hemoglobin or heme)Hemo

5、globin is present in red blood cells and is a kind of protein containing iron.Adult male is 120 ~ 160g/LAdult female is 110 ~ 150g/L,Measurement parameters of blood cell analyzer,Red blood cell RBCWhite blood cell WBC

6、Platelet PLTHemoglobin HGB,Red blood cell RBCWhite blood cell WBCPlatelet PLTHemoglobin HGB,一、The development history of blood cell analyzer,In 1956, the American scientist W.H.Coulter patented technology for the us

7、e of the resistance method to count the number of particles in the blood cell count to be successful.The principle is based on the nature of the non conduction of blood cells, the blood cell count and the volume of bloo

8、d cells were detected by the blood cell count and volume measurement. The method is called the resistance method or the Kurt principle.,,國外廠商：日本SYSMEX美國beckman-coulter日本KOHDEN美國DANAM瑞典BOULE美國BAYER美國ABBOTT,國內(nèi)廠商：南昌

9、特康南昌百特深圳邁瑞桂林百利特,1. 庫爾特計數(shù)原理,庫爾特原理（coulter principle） ：,二、血細(xì)胞計數(shù)檢測原理,Coulter principle,When the electrolyte is suspended in the electrolyte, the same volume of electrolyte is replaced by the electrolyte through the hole,

10、 and the electric resistance of the two electrode is changed, and the potential pulse is generated. The size and frequency of the pulse signal is proportional to the size of the particles and the number of the particles.

11、,二、血細(xì)胞計數(shù)檢測原理,儀器是如何進(jìn)行細(xì)胞分群的呢？,儀器除給出細(xì)胞數(shù)據(jù)結(jié)果外，同時提供出細(xì)胞體積分布圖形，這些可以表示出細(xì)胞群體分布情況的圖形被稱為直方圖它可以顯示出某一特定細(xì)胞群的平均細(xì)胞體積、細(xì)胞分布情況和是否存在明顯的異常細(xì)胞群直方圖是由測量通過感應(yīng)區(qū)的每個細(xì)胞脈沖累積得到的，根據(jù)庫爾特原理可以在計數(shù)的同時進(jìn)行分析測量。,二、血細(xì)胞計數(shù)檢測原理,Relationship between histogram and puls

12、e signal,二、血細(xì)胞計數(shù)檢測原理,三、Structure of ACT diff Coulter blood cell analyzer,The volume and number of blood cells were measured by using the principle of Kurt resistance, and the hemoglobin was detected by using the principl

13、e of monochromatic light color, and the three classification of white blood cells was obtained according to the distribution of blood cell volume.。35 to 50 samples per hour. WBC,RBC,HGB,PLT,LY,MO,GI,,三、Coulter ACT diff血

14、細(xì)胞分析儀的結(jié)構(gòu),Card slotCards and reagents for the corresponding batch should be,,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),Counting software card,,Positive internalSuction needleReaction tankPhotoelectric tubeSolenoid valveScan tube

15、Vacuum isolation chamber,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),,Reaction tank,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),,Right sideCreeping pumpDilution liquid storage tankNeedleSolenoid valveFilterAir pump,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),,Pi

16、nch valve,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),,Valve core,,Control board,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),,Instrument structureGas path: vacuum - pumping waste liquid, drying; compressed air - producing bubblesLiquid path: sample,

17、hemolytic agent, diluent, waste liquid and cleaning liquidCircuit: control of gas path, counting and calculation.,三、Coulter ACT diff血細(xì)胞分析儀的結(jié)構(gòu),,Piping working process:SampleWipe the needle into the external and drySam

18、ple dilution such as WBC reaction tankThe two dilution sample was injected into the RBC reaction tankIn WBC reaction tank, the injection of hemolytic agentWBC count (control), PLT count, HGB count (photoelectric metho