冬小麥矮稈突變體DC20的蛋白質(zhì)組學分析.pdf

1、新疆農(nóng)業(yè)大學碩士學位論文冬小麥矮稈突變體DC20的蛋白質(zhì)組學分析姓名：趙明輝申請學位級別：碩士專業(yè)：作物栽培學與耕作學指導教師：章建新20090601ProteomicsAnalysisofaDwarfMutantDC20inWinterWheatAbstractTheGA3sensitivityandgeneticanalysisofwheatdwarfmutantDC20，whichwasinducedfromadvancedlin

2、eD6—3by185Gymixedparticlefield，werestudedinthispaperAndproteomicstechnologywasusedtocharacterizethedifferentiallyexpressedproteinsofdwarfmutantDC20inthedifferentdevelopmentalstagesofseedling，shooting，bootingandheadingsta

3、ge，respectivelyThiswillleadtounderstandthefunctionofdifferentiallyexpressedproteinsrelatedwithdwarfphenotype，tevealthemolecularmechanismofmixedparticlefieldmutagenesisininducingdwarfmutantTheresultsshowedthatthedwarfmuta

4、ntDC20WasGA3sensitive，andthedwarfphenotypewascontrolledbyasinglerecessivegeneTheproteomicsanalysisrevealedthefollowing：intheseedlingstage，thereexisted47differentiallyexpressedproteins，amongthem11proteinsupregulated，13pro

5、teinsdown—regulated，and23proteinsdidnotexpress；intheshootingstage，therewere24differentiallyexpressedproteins，amongthem10upregulated，10downregulated，4proteinsdidnotexpress；inthebootingstagetherewere11differentiallyexpress

6、edproteins，amongthem5up—regulated，5down—regulated，1proteinsexpressedonlyinDC20；intheheadingstageexists9differentiallyexpressedproteins，1upregulated，5downregulated，3proteinsdidnotexpressed76differentiallyexpressedproteins

7、wereselectedtoCalTyoutMALDI—TOF／MS40mastproteinswereidentifiedandclassifiedinto7groups，ie，energymetabolismproteins，substancemetabolismproteins，celldivisionandgrowthproteins，cellstructureproteins，resistanceassociatedprote

8、ins，proteintargetingandstorageproteins，andunknownfunctionproteinsEnergyandsubstancemetabolismproteinsaccountedfor52％，implyingthatthedwarfphenotypeWasduetothehamperingofenergyandsubstancemetabolismofthedwarfmutantDC20inth

9、egrowthdevelopmentInthebootingstage，animportantprotein(GA2oxidase2)directlyinvolvedinGAbiologicalmetabolismWasfutheridentifiedItup—regulatedinDC20inbootingstage，mightbereducthecontentofactiveGAintheplantwhichcouldnotmeet

10、thelevelofnormalgrowth，directlyleadtotheexpressionofdwarfphenotypeItWasconcludedthattheremaybeacomplexproteomeregulationnetworkintheexpressionofdwarfphenotypeinducedbymixedparticlefieldThedifferentiallyexpressedproteinso

11、fdifferentstagesplayedrolessinglyorsynergiclyintheformationofdwarfphenotype，andfinallyaffecttheplantgrowthandheightTheresultswerehelpfulltofurtherclarifythemolecularmechanismofthedwarfinwheatKeywords：winterwheat；dwarfmut