3N-2N水稻雙胚苗及其雜交后代比較分析.pdf

1、四川農業(yè)大學碩士學位論文3N2N水稻雙胚苗及其雜交后代比較分析姓名：張鵬申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：汪旭東吳先軍20030501AbstractTriploidandtetraploidderivedfromtwin—seedlingpopulationscrossedwithsomecommondiploid，andsomestableprogenieswereobtainedinsecondfilialgenera

2、tioninRiceResearchInstituteofSichuanAgriculturalUniversityForyearsresearchesindicate，thiskindofearlygenerationstablephenomenononlypresentonfilialgenerationinvolvingpolyploidWhereasinearlygenerationofhybridofdiploidcorres

3、pondingthepolyploidintwoseedlingthatcrosswithcommondiploidlthiskindofspecificphenomenonneverappearedbynowInthisstudywecontrastandanalysefivecoupleofplantsof3Nand2NrespectivelyderivedfromtwinseedlingwithSSR，inordertoascer

4、tainifgene(s)orfactor(s)promptingearlygenerationstabilitywasproducedinthegenomeof3Ninthecourseoftwin—embryo，andanalysefurthercombinedwiththestabilityoffilialgenerationof3Nand2Nplants。Themainresultsarefollowing：國Fivepairs

5、of3N2Ntwin—seedlingwereanalysedbySSR。Andpolymorphismdoesnotpresentat425primerslociSotheresultisconferredthattherearenotdifferenceinDNAsequenceon3Nand2NplantsoftwinseedlingThedifferencesofphenotypeandbehaviorbetweenthemma

6、yberesultedfrompely≯loidiz#tionanddosageeffectsForcontrastivemethodwassinglenessfurtherresearchwereneeded國3Nplantsfromtwinseedlingactasfemaleparent。crossedwithcommondiploidasmale，267Flhybridseedswereobtained。Percentageof

7、seedsettingfromallcombinationswasabout0。7％。Andbytubeculture52seedsofFtgerminatedAllseedlingswereplantedinfieldin2001ThemostwerediedandnineFIhybridsurvived，AllofthemwereaneuploidandonlyfiveplantswerepartialfruitingTheseed

8、sfromeachpanicleoftheseFIhybridswereharvestedrespectivelyAtthesametime，F(xiàn)hybridsof2NX2Nwereplantedinfieldandrevealednormalphenotype，TheirF2populationsappearedsegregationandnostablepopulationsappearedItwasmaybedirectreason