30502.擬南芥ft10突變體與野生型差異表達(dá)蛋白質(zhì)的初步研究

1、廈門大學(xué)碩士學(xué)位論文擬南芥ft10突變體與野生型差異表達(dá)蛋白質(zhì)的初步研究姓名：陳美珍申請學(xué)位級別：碩士專業(yè)：發(fā)育生物學(xué)指導(dǎo)教師：朱學(xué)藝黃濤20090501AbstractAbstractItiswellknownthatFTgeneplaysanimportantroleinphotoperiodpathwayregulatingfloweringinArabidopsisThusthecomparativeresearchesbetw

2、een缸mutantandWTmightprovideasoundbasisforbetterunderstandinginthemolecularmechanismsofplantfloweringTwodimensionalelectrophoresisandMALDI—TOFMSanalysisareusedtOanalysethedifferentlyexpressedproteinsbctwegnthe曩10mutantand

3、itswildtypeofArabidopsisItishopedthattheseexperimentalresultsmightbehelpfultOprovideinformationforthemolecularmechanismsofplantfloweringThemainresultsshow勰follows：(1)Tooptimizetwodimensionalpolyacrylamidegeleleetrophores

4、is(2DEE)systemfortheseparationandquantificationofplantproteinspecies，suitable2DEEsystemwasdevelopedbyselectingdifferentcomponentsandconcentrationsinisolationsolutionsandlysisbuffersSampleWaspreparedbyusing0IMammoniumacet

5、ateinmethanol(NFhAc／methyl)Theisolatedproteinswerelysisedinlysisbufferconsistingof8Murea2MThiourea，4％CHAPS，65mMD1vr，2％Ampholineandlmmol／LPMSFTheseparatedproteinsonthe2DEgelweredetectedbyCoomassiebrilliantblueR250staining

6、IthashighqualityandquantityseparationofleaftOtalproteinsinthis2DEimage(2)34differentiallyexpressedproteinsintheleaftOtalproteinsbetweenfilOmutantanditswildtypeweredigestedingelbytrypsinandanalyzedbymatrixassistedlaserdes