18813.產(chǎn)脂肪酶微生物的篩選及其全細(xì)胞固定化研究

1、南開(kāi)大學(xué)碩士學(xué)位論文產(chǎn)脂肪酶微生物的篩選及其全細(xì)胞固定化研究姓名：吳豐鵬申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：環(huán)境科學(xué)指導(dǎo)教師：沈伯雄201205Abstract_———————————————————————————————————————一AbstractUnderthepressureofcrudeoilexhaustionanddemandincreasing，todevelopthebiodieselisofstrategicimporta

2、ncetosustainabledevelopmentofeconomylighteningtheenvironmentalstress，controllinggreenhousegasemissionandconstructinglowcarbonsocietyComparedtoothermethodstosynthesizebiodiesel，Usingbiologicalenzymehasmanyadvantages，sucha

3、smildreactionconditions，smallamountofalcohol10Wcontentofwaterandfreefattyacidinfeedstock，nonpollutingemissionandSOonButfleelipaseiseasytoclusterandveryunstableaswellasrunningoffseriouslybeingdifficulttobeseparated，whichm

4、akeslipasemethodcosttoohighlySOtechnologyoflipaseimmobilizationhasdevelopedtosomeextentHoweverthehighcostofthistechnologyrestrictsthewidespreadapplicationinthecommercialprocessTherealeabundantmicrobialresourcesinsoil，whi

5、lemostofmicrobialCansecretlipase，SOsomelipasesecretingmicrobialisfirstselectedfromsoilspollutedbyfatorplantoil，ofwhichtheenzymaticactivityandbiologicalfeatureswerestudiedinordertosearchakindofmicrobialsuitableforproducin

6、gbiodieselfromplantoilandthereforetolowerthecostofbiodieselindustrybyenzymeVictoriablueisusedtoselectamicrobialwithgoodlipase—secretingactivityThismicrobialisidentifiedtobeEnterobacterspby16SrRNAmolecularbiologymethodThe

7、lipaseactivityofthemicrobialisstudiedinthistextTostudytheimpactofculturetimeandtemperatureonlipasesecretingactivitythebestcultureperiodof4daysandbestculturingtemperatureof32。CaledeterminedbythepHchangingfromsubaciditytoa

8、lkalescencethentostrongbasicitylipaseactivityfirstrisesandthendeclines，determiningthebestpHof85Freeenzymeiseasytogathertogetherextremelyunstableaswellasdifficulttoseparateandlosttoomuch，whichincreasesthecostofenzymaticpr