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1、Y773403分類譬霄級(jí)單位代碼:10019學(xué)號(hào):B02066‘專峨巖嚏夭等博士學(xué)位論文生姜原生質(zhì)體培養(yǎng)與體細(xì)胞變異ProtoplastCultureandSomaticVariationofGinger(ZingiberofficinaleRosc)(國家科技攻關(guān)資助項(xiàng)日60|1002)(農(nóng)業(yè)部農(nóng)業(yè)結(jié)構(gòu)調(diào)整項(xiàng)目040602B)研究生:酆甚垡指導(dǎo)教師:一毯拯豎熬援中請(qǐng)學(xué)位門類級(jí)別:盛堂埴專業(yè)名稱:蘊(yùn)苤堂研冤方向:益苤生理生盆工生塑堂所存
2、學(xué)院:丞堂生生物整盔堂逮二零零五年五月中國農(nóng)業(yè)大學(xué)博士論文摘受AbstractGinger(Zingiberofficma缸R(shí)ose)isacultivarproducingrhizomesofthefamilyZingiberaceaeItismostlydistributedintheAsiantropicalandsubtropicsregionAndSouthAfrica,NorthAmericaandAustraliaalso
3、plantgingersGingersarerichinvolatileoilsandwidelyusedasacondiments,dyes,beverage,medicineandvegetableGingersannuallypropagatethemselvesbyrhizomeswithlowproliferationrateTheyareheavilyattackedbysoilboropathogensAndthequal
4、ityofrhizomesdegeneratesforthelongtermagamicpropagationGingersseldomflowersandhaveseeds,soitisdifficulttocultivatenewvarietythroughcrossbreedingInthispaperinvitrotissueculturetechniqueswerecarriedoutincludingsomaticandem
5、byogeniccallusinductionembryogenicsusppeosioncultureandprotoplastculture1Somaticembryogcniccalliof4gingergenotypeswereproducedSomaticembryogeniccalluswasinducedfromgingershoottipsonMSmediumsupplementedwith10mg/L2,4Dand02
6、mgU1KTThecallus(about1Scmindiameter)weretransformedintotheMS02mg‘L‘24一D50mgL1BA3%socrose07%ag礬andshootsandrootswereformedAndshootsdevelopedintocompleteplantletswithrhizomesonsolidMSmediumsupplementedwith30ragL1BAand01mgo
7、L1NAAand6%sucroseTheeffectofphytohormonesonembryogeniccallusformationandredifferenciationwasdiscussedTheeffectofsucroseonrhizomeformationwasresearched2Somaticembryogenicsuspensionculmresof4gingergenotypcswere:established
8、Fromwhich,regeneratedplantletswereobtainedTherelationshipbetweenthedryweightfDw)ofsuspensionculturesandpHchangesinmediumwasalsodiscussedTheeffectsofinoculationandAgN03Oilthegrowthofembryogenlcsuspensionswerealsodiscussed
9、TheoptimumconcentrationofinoculationandlevelofAgN03were10%and60mgL~respectively3Protoplastswereisolatedfromembryogenicsuspensionsincubatinginenzymesolutioncontaining40%celluloseOnozukaR10,10%maceroymeR10,01%PectolyaseY23
10、,05%CaCl2,01%IVIESand11%mannit01Theenzymemixturewerekeptinastationarypositionfor6handshaken(60rpm)for6hat27℃Theyieldandviabilitywere627X1069—and907%respectivelyPurifyingprotoplastswereculturedinitiallyinshallowliquidlaye
11、rs4Thechromosomenumbersofembryogeniccallusderivedplants,embyogenicsuspension—derivedplantsandprotoplastderivedplantsWere22,equaltotheirparentplantsTherewemdifferencesofmorphologicalcharactersbetweeninvitroplantsandpsxcnt
12、plantssuch鵲leafthick,leafcolorandLeafindexTheRAPDanalysisofregeneratedplantsindicatedrelativelysomaticvariationThechromosomenumberdeterminationandRAPDanalysisofembyogeniccallusandembryoenicsuspensionderivedandprotoplastd
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