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1、湛江海洋大學(xué)碩士學(xué)位論文紅笛鯛(Lutjanussanguineus)性腺發(fā)育和血清性類固醇激素的研究姓名:楊春玲申請(qǐng)學(xué)位級(jí)別:碩士專業(yè):水產(chǎn)養(yǎng)殖指導(dǎo)教師:劉楚吾20050430StudiesongonaddevelopmentandserllmsexualsteroidsofLutjanu$sanguineusABSTRACTGonaddevelopmenttheannualchangesofserunlsexualsteroidsa
2、ndtheseasonaldifferencesofserumsexualsteroidsbeinginjectedhormonesanddrugsilILutjanussanguineuswerestudiedExperimentone(EXPI)studiedthegonaddevelopmentofLsanguineus111eresultsshowedasfollowed:thetesticulardevelopmentwent
3、throughthewholedevelopmentcourseofspermatogonlumspermatocyteI,spermatocyteII,spermatidandspermButincombinationwiththeanatomicalcharactertestisonlydevelopedtotheearlystageⅣOogoniumoucyteofthephaseIIoocyteofthephaseⅢwereob
4、servedinthefemaleLsanguineusButcombiningwiththeanatomicalcharacterovaryonlydevelopedtothestageⅡGonadosomaticindex(GSI)duringtheannualreproductivecycleWasmeasuredtheresultsshowedthatGSImaintainedathigherlevelinMarchandApr
5、il,reachedthehighestlevelinMa孔droppedinJulyhadalessapexinAugustandtendedtowardsstabilizationinIatterseveralmonthsiIIthefemaleVarietyofthemaleWasbasicallysimilarwiththefemaleInadditionwealsodiscoveredthathepatosomaticinde
6、x(HsI)andGSIpresentednegativerelativityinthefemale,butthisrelationdidn’texistinthemaleEXP2measuredIevelsofserumsexualsteroids,including17BestradioI(E2),testosterone(13,progesterone口)inLsanguineususingradioimmunoassay(glA
7、),andresearchedtheirvarietyregulationduringtheannualreproductivecycleVarietyregulationofE2wasthatthefemaleandthemale厶sanguineusweresimilarduringtheannualreproductivecycleThatisbothwereintheIowerlevelsfromMarchtoOctoberha
8、dalittleapexonAprilandwentupcontinuouslysinceNovemberRegardingT,themaleLsanguineushadacertainregulationThatis,thelevelsofTwereatthehigherlevelsfromMarchtoSeptemberthendescendedandalwaysmaintainedatthelowlevelMoreoverleve
9、lsofTinthefemaleLsanguineusfluctuatedupanddownandhadnoobviousapexV撕etyregulationofPshowedthatthelevelsofPinboththefemaleandthemaleweremostIyalikeduringtheannualreproductivecycleThatis,thelevelsofPwereatthehigherlevelsinA
10、prilandMa弘andthenfluctuatedatthelowerlevelsEXP3measuredthelevelsofserumsexualsteroidswithtimeafterbeinginiectedhormonesanddrugsinLsanguineusindifferentseasonsusingRIA11lcfollowingresultswerefound:(1)ChangesofsenImE2wered
11、ifferentindifferentseasonsbeinginjectedhormonesanddrugsInMayinjectingL玨tHA3,HCGLHRHA3HCGDOMandLHRHA3DOMaIlincreasedlevelsofE2atdifferentdegree,andresultswerebetterinjectingLHRHA3HCGandLHRHA3DOMthan兩ectingsolelyLHRHA3,HCG
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