中國(guó)荷斯坦奶牛催乳素基因和微衛(wèi)星DNA多態(tài)性與產(chǎn)奶性能的相關(guān)分析.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文中國(guó)荷斯坦奶牛催乳素基因和微衛(wèi)星DNA多態(tài)性與產(chǎn)奶性能的相關(guān)分析姓名：徐華申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物遺傳育種與繁殖指導(dǎo)教師：孫少華20040618AssociationsbetweenProlactinGeneandMicrosatelliteDNAPolymorphismsandMilkProductionTrajtsofHolsteincattleinChinaMaster：XuHuaSupervisor：

2、SunShaohuaMajor：AnimalGeneticsBreedingandReproductionScienceAbstractSelectionofhighqualitycattlesisprimaryapproachforincreasingcattlesproductionperformanceanddairyfarmseconomicbenefitTraditionaljudgingofexteriorandproduc

3、tionperformancehavebiggerlimitationbecauseofenvironmentalfactorinfluenceandtime，sexconfinement，butselectionofmoleculegeneticmarkercombiningproductiontraitfordairygeneticqualityishighaccuracymethodtoconquertheselimitation

4、sThisexperimentselects130HolsteindairycattlesinthedairyfarmbelongingtoShiJiazhuangSanluGroupandtheHonglidadairyfarminRongchengtotallyAppliedPCR—RFLPandmicrosatelliteDNApolymorphismdetectiontechniqueProlactin(bPRL)andfour

5、microsatellitelociHolsteincattleareanalysedforDNApolymorphism。EffectsofbPRLandmicrosatellitelocionmilkperformancewereexaminedusingleastsquaresmodelFrequencyofvariantsinPRLwere：PRL(A)07538，PRL(a)02462Forthegenotypefrequen

6、cyofPRL，thegenotypeofAAisO5462，Aais04154，aaisO0385ThegenefrequencyandgenotypefrequencyofPRLwereanalysedusingchi—squaretestTheresultshowedthattheHolsteindairyCattleliesinHardyWeinbergequilibriumstateTheheterozygsity，numbe

7、rofeffectivealleles，andpolymorphisminformationcontentwereO3711，1，5901，O3022，respectivelyThePICshowedthemediumpolymorphismThisexperimentmadeuseoffourmicrosatelliteloci(ILSTS103，ILSTS96，CSSM66，BM6425)analyzingdairycattleco

8、nstructionandvariationwithinheritThegenefrequencyandgenotypefrequencyoffourmicrosatellitelociwereanalysedusingchi—squaretestTheresultshowedthattheHolsteindavycattleliesinHardy—WeinbergequilibriumstateThroughChi—squareind

9、ependencetest，theCSSM66andBM6425locatingontheNo14thchromosometogether，ismutuallyindependentontheheredityThegenefrequencyandgenotypefrequencyoffourmicrosatellitelociwereanalysedusingchisquaretestTheresultshowedthattheHols

10、teindairycattleliesinHardy—WeinbergstateThenumberofalleles，rangeofthepolymorphisminformationcontent，numberofeffectiveallelesandtheheterozygsityinthepopulationwere5～11，O78～O88，483～819and079～088Theseresultedshowthatfourmic