erβperβ在小鼠發(fā)情周期和早期妊娠卵巢中表達與分布的研究

1、東北農(nóng)業(yè)大學碩士學位論文ERβpERβ在小鼠發(fā)情周期和早期妊娠卵巢中表達與分布的研究姓名：鄭可佳申請學位級別：碩士專業(yè)：發(fā)育生物學指導教師：嚴云勤20090620東北農(nóng)業(yè)大學理學碩士學位論文IIAStudyonERβpERβExpressionDistributioninMouseOvariesDuringOestrousCycleEarlyGestationalPeriodAbstractEstrogenwhichhasimptant

2、effecttothemammaliangenitalsystemendocriniumisoneofthechiefendogonadalhmones.Itisveryimptanttoregulatethedevelopmentofovaryfolliclesmaturityffemalemammal.Withestrogenrecept(estrogenreceptER)estrogencanfmhomodimerheterodi

3、merwhenthedimmersarephosphylatedtheyactivatethepromoterofestrogensensitivegeneswhicharerelatedwithnucleusregulationthentheybringintobiologicaleffect.ERbelongstonuclearreceptsuperfamilyincludingestrogenreceptalpha(ERα)est

4、rogenreceptBeta(ERβ).Theirexpressionshowtissuespecificitybothofthemexpressinmouseovarybuttheexpressionphysiologicalfunctionsaredifferentbetweenthem.Recentllyexperimentsshowedthatovariesfromrodentshumanthetranionlevelprot

5、einexpressionquantityofERβarebothmehigherthanthatofERα.InERβgeneknockedoutmousethefunctionofovariesarebasicnmaltheovarianfolliclecangrowtoantralfolliclesbutthefrequencyamountofovulationwereobviouslydecreased.Theseshowedt

6、henecessityofERβtokeepthephysiologicalfunctionofovary.ButpresentstudysonexpressiondistributionofERβinmammaliangenitalsystemisveryfewespeciallythisofpERβwhichistheactivationfmofERβ.Therefeitisnecessarytoresearchtheexpress

7、iondistributionofERβanpERβwiththeexperimentalmodelofthemouseovariesduringoestrouscycleearlygestationalperioddiscusstherelationshipbetweenthetwoactiveconditionsofthemoleculefunctionperfminginovary.Thisstudycanoffertheyexp

8、erimentevidencestotheresearchofERsactionmechanisminovaryitcouldmakepeopleknowERβpERβfurther.MeoverthereissomecrelationbetweenERβovariancancerstudiesshowthattheexpressionofERβinovariantumarelessthanthatinnmalovary.Sothest

9、udycanofferevidencetopreventdiagnosetreatthetumofovaries.InthisexperimenttheobjectwasovariesfromadultmouseweusewesternblottechniqueimmunohistochemistrymethodtostudyresearchexpressiondistributionchangesofERβpERβduringoest

10、rouscycleearlygestationalperiod(1~11d).Theexperimentalresultindicatedthat:1.TheresultsduringoestrouscycleofwesternblotshowedthatinmouseovaryERβpERβwerehighlyexpressedatoestrusbuttherewasnosignificantdeviationatotherperio