仔豬復(fù)合益生乳酸桿菌制劑及其作用機(jī)理的研究.pdf

1、中國(guó)農(nóng)業(yè)大學(xué)博士學(xué)位論文仔豬復(fù)合益生乳酸桿菌制劑及其作用機(jī)理的研究姓名：黃滄海申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)指導(dǎo)教師：李德發(fā)譙仕彥20030601AbstractLactobacillistrainswereisolatedfromgastrointestinal(GI)tractmucosaandscreenedouttodevelopacompIexiactobacillipreparation，whichwouldal

2、leviatepig／etweanings“essTwoanimalexperimentswereconductedtostudytheactionmodeofthepreparationinpigletsOnthebasisofstresstoleranceandprobioticcharacteristics，Lactobacillusgasseriwasselectedfrom1220strainsinstomachandLreu

3、terifromduodenum，LacidophilusfromjejunumandLye門(mén)竹eHf啪fromcolon，werealsosingledoutfrom5789intastina]strainsThebiologicalcharacteristicsoffourstraimsoflactobacillusweredeterminedandfermentationparametersoftheselactobacilliw

4、ereoptimizedbymegnsofuniformdesignLactobaeillisurvivabilityafter75Ctreatmentfor15min，pH20treatmentfor6handstoragefor1month，respectivelyweremeasuredLgasserisurvivabilityafter商℃，storage，andpH20treatmentwas72％，378／0133％，res

5、pectivelyandfermentationparameterswereasfoflows：culturetime20h，glucose110g，L，sucrose60grL，peptone30e：，yeastextract30g／L，andammoniumcitrate2g，LLreuterisurvivabilityafter75℃，storage，andpH20treatmentwas313％，855％，344％，respec

6、tivelyandfermentationparameterswereasfollows：culturetime20h，glucose10g，L，sucrose60∥L，peptone30g，L，yeastextract2嘰，andammoniumcitrate2g，LLaeidophilussurvivabilityafter75℃，storage，andpH20treatmentwas625％，59％，695％respectivel

7、yandfermentationparameterswereasfollows：culturetime20h，glucose110啦，sucrosetO班，peptone5仉，yeastextract30以，andammoniumcitrate，12班三fermentumsurvivabilityafter75“C，storage，andpH2，0treatmentwas451％，784％，809％，respectivelyandfer

8、mentationparameterswereasfollows：culturetime20h，glucose10g，L，sucrose60g，L，peptone30g，L，yeastextract30e／L，andammoniumcitrate12g，LTheoptimalratiosoffourlactobacillibacteriaincomplexpreparationweredeterminedinuniformdesignT

9、heproportionoflactobacilliisasfollows：上gasseri：Lreuteri：Lacidophilus：L枷nentum=2：3：5：2Twelvepigs(765110kgBW)，weanedat28d，wereallocatedrandomlyinto2groupsOnegroupwasprovidedwithwatercontainingthepreparationandtheotherwitht

10、apwaterascontrolbeforepigletwasorallyadministeredwithEcolfonthe8thdInthefollowing7ddiarrheaindexanddiarrheaincidenceweredecreasedby66and691％respectivelyinlactobacillitreatedgroupThelactobacillisignificantlydecreasedEcoli

11、coilfoITasandaerobescountsbutincroasedIactobacilli，bifidobacteriaandanaerobescountsindigestaandmucosaofmostsectionsofGItractThirtysixpigletsweanedat28d(765109kgBW)wereallocatedrandomlyintotwogroupswithsixpenspergroupandt

12、hreepigsperpenHalfofpigletswerefedanonmedieatedbasaldietwithlactobacillipreparationandtheotherwith50mg爪gcarbadoxascontr01ThelactobacillipreparationsignificantlyimprovedpigletsADGby302％andADFIby239％comparedwithearbadox(Po

13、05)duringthefirst2wksafterweaningFromd15tod21improvedgrowthperformancewasnotobservedin1actobacinitreatedgroupThelactobacillisignificantlyincreased1aetobacilli，bifidobacteriaanaerobes，最colicoilfoITIlS，andaerobescountsindi

14、gestaandmucosaofmostsectionsofG]tractDigastibilityofnitrogenandtotalphosphoruswasonlysignificantlyincreased(P005)BUNwasdecreasedinlactobaeilligroup妒0oDTheresuItsfromtheseexperimentsshowedthatthecomplexlactobacilliprepara