白介素-18在大鼠視網(wǎng)膜新生血管形成中作用的實(shí)驗(yàn)研究.pdf

1、石河子大學(xué)碩士學(xué)位論文白介素18在大鼠視網(wǎng)膜新生血管形成中作用的實(shí)驗(yàn)研究姓名：閭雯娟申請學(xué)位級別：碩士專業(yè)：眼科學(xué)指導(dǎo)教師：唐寧201103II論文類型：B（應(yīng)用研究）AbstractObjective：Objective：ToinvestigatetheeffectsofIL18onretinalneovularizationtheexpressionofVEGFonmice.MethodsMethods：Fiftysevendayo

2、ldneonatalSDmicewereromlydividedintofivegroups:controlgroup、Naclgroup、hyperxiaexperimentalgrouplowconcentrationexperimentalgrouphighconcentrationexperimentalgroup.Naclgroup、hyperxiaexperimentalgrouplowconcentrationexperi

3、mentalgrouphighconcentrationexperimentalgroupexposedto(75020)mlLoxygentoestablishmodelsofretinopathyneovularizationffivedays.Themiceinlowconcentrationexperimentalgrouphighconcentrationexperimentalgroupwererespectivelytre

4、atedwithintravitrealinjectionofinterleukin18(1μL)10ngμL50ngμL.ThemiceinNaclgroupintravitrealinjectionofNacl(1μL).Theanimalswerekilledatthetwentyfirstday.Countthenumbersoftheendotheliocytenucleiofneovularizationwhichbroke

5、throughtheretinalinnerlimitingmembranebytheHEstaining.TheexpressionofVEGFCD34atlayersofretinawasdetectedbyimmunohistochemistry.Results:Results:①Thenumbersofendotheliocytenucleiofneovularizationsolittleincontrolgroup（1.40

6、0.70），butinhyperxiaexperimentalgroup（14.72.26）lowconcentrationexperimentalgroup（10.11.52）highconcentrationexperimentalgroup（6.501.27），therearemuchmeendotheliocytenucleithelowconcentrationexperimentalgroupmethanhighconcen

7、trationexperimentalgroup.②TheexpressionofVEGFwasfoundatalllayersofretinaineachgroupsignificantlyincreasedinhyperxiaexperimentalgroup（3.500.71）Naclgroup（3.700.48）relativelydecreasedinhigh（1.900.74）low（2.600.52）concentrati

8、onexperimentalgroupwastothelowestincontrolgroup.ThereweresignificantdifferencesflowhighconcentrationexperimentalgroupcomparedwithhyperxiaexperimentalgroupNaclgroup(bothP0.05)muchme，thestatisticaldifferencewasfoundbetween