RNA干擾CD147的表達(dá)對人胃癌細(xì)胞株SGC7901增殖侵襲的影響及相關(guān)信號(hào)通路的研究.pdf

1、摘要學(xué)位論文獨(dú)創(chuàng)性聲明本人鄭重聲明：所提交的學(xué)位是本人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作和取得的研究成果。本論文中除引文外，所有實(shí)驗(yàn)、數(shù)據(jù)和有關(guān)材料均是真實(shí)的。本論文中除引文和致謝的內(nèi)容外，不包含其他人或其它機(jī)構(gòu)已經(jīng)發(fā)表或撰寫過的研究成果。其他同志對本研究所做的貢獻(xiàn)均己在論文中作了聲明并表示了謝意。學(xué)位論文作者簽名：7萬、而每El期：山，歲六W學(xué)位論文使用授權(quán)聲明研究生在校攻讀學(xué)位期間論文工作的知識(shí)產(chǎn)權(quán)單位屬南京師范大學(xué)。學(xué)校有權(quán)保存本學(xué)位論文

2、的電子和紙質(zhì)文檔，可以借閱或上網(wǎng)公布本學(xué)位論文的部分或全部內(nèi)容，可以采用影印、復(fù)印等手段保存、匯編本學(xué)位論文。學(xué)?？梢韵驀矣嘘P(guān)機(jī)關(guān)或機(jī)構(gòu)送交論文的電子和紙質(zhì)文檔，允許論文被查閱和借閱。(保密論文在解密后遵守此規(guī)定)保密論文注釋：本學(xué)位論文屬于保密論文，保密期限為年。學(xué)位論文作者簽名：亍孑、融湯El期：u，|，卜M指導(dǎo)教師簽名：易第乞Et期：L，≯，矽AbstractAbstractGastriccanceriSthefourthmos

3、tcommoncancerworldwide，anditiSthesecondmostcommoncauseofcancerdeathDespitemuchresearch，wedonotyethavedrugsadequmefortreatinggastriccancerAbetterunderstandingofthemolecularmechanismsdrivinggastrictumorigenesisiscrucialand

4、willprovideenormousbenefitsindevelopingnewandeffectivetherapeutictreatmentsforgastriccancerCD147isaheavilyglycosylatedtypeItransmembraneglycoproteinthatbelongstotheIgsuperfamilyCD147hasbeenimplicatedinnumerousphysiologic

5、alandpathologicalactivitiesItisusuallyexpressedatlowlevelsinmostnormaltissues，butishighlyupregulatedduringvariouscancel“progressionCD147inducesseveralMMPsinstromalfibroblastsandendothelialcellsaswellasintumourcellsthemse

6、lvestopromotetumorgrowth，invasion，metastasisInthisstudyweinvestigatethepossibleroleofCD147intheprogressionofgastriccancerbYusingRNAinterference(RNAi)technologyShonhairpinRNA(st刪A)expressionvectorstargetingCD147wereconstr

7、uctedtosilenceCD147，andtheexpressionofCD147WasmonitoredbyquantitativerealtimeRTPCRandWesternblotCellproliferation，theactivitiesofMMP一2andMMP9，theinvasivepotentialofSGC7901cellsweredeterminedbyMTTgelatinzymographyMatrigel

8、invasionassayandMTTrespectivelyThephosphorylationandnon—phosphorylationofthemitogenactivatedproteinkinasesERKl／2，P38andJNKwereexaminedbyWesternblotAdditionallytheERKl／2inhibitorU0126wereusedtoconfirmthesignalingpathwayin

9、volvedinCD147mediatedSGC7901progressionTheresultsrevealedthatCD147silencinginhibitedtheproliferationandinvasionofSGC7901cells，anddownregulatedtheactivitiesofMMP一2andMMP9andthephosphorylationoftheERKl／2inSGC7901cellsERKl／

10、2inhibitorU0126decreasedtheproliferation，andinvasionofSGC7901cells，anddownregulatedtheMMP2andMMP9activitiesTheseresultssuggestthatERK1／2pathwayinvolvesinCD147mediatedgastriccancergrowthandinvasionThesefindingsfurtherhigh