類缺血再灌注條件下Midkine因子對(duì)神經(jīng)干細(xì)胞增殖、分化、凋亡的影響.pdf

1、佳木斯大學(xué)碩士學(xué)位論文類缺血再灌注條件下kine因子對(duì)神經(jīng)干細(xì)胞增殖、分化、凋亡的影響姓名：張鵬申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：神經(jīng)病學(xué)指導(dǎo)教師：張曉梅朱曉峰20080501AbstractObjective：TheresearchisdoneforthepurposeofobservingthefunctionofMKonratneuralstemcellsculturedinvitroandtheeffectofMidkineonthene

2、uralstemcells’proliferation，differentiationandapoptosisinischemia—reperfusion—likeconditionMethods：Usingthecellcounting，theimmunohistochemistryandimmunofluorescencemethodexaminetheinfluenceofdifferentdensityMidkineonprol

3、iferation，differentiationandapoptosisoftheneuralstemceiisculturedinvitroSelectthemostsuitabledensityMidkinewhoseeffectontheneuralstemcellsisobviousandcontinuethenextstepEstablishingischemia—reperfusion—likeconditioninvit

4、rojusingthecellcounting，theimmunohistochemistryandimmunofluorescencemethodtoobservetheeffectofpurenervestemcellgroupandthesuitabledensityMKgrouponproliferation，differentiationandapoptosiSofneuralstemcellsinreperfusionaft

5、erischemia05h，lh，3h，6hconditionResults：TheMKlOng／mlisthebestdensitytopromoteproliferation，todifferentiateneurondirection，teduceapoptosisthegrowthrateofMKgroupcellishigherthanthatofthepureneuralstemcellgroupinischemia—rep

6、erfusion—likecondition：ThenumberofNSEpositivecellsintheMKgroupishigherthanthatofthepurenervestemcellgroupineverytimeduringischemia；ButThenumberofGFAPpositivecellsintheMKgroupis10werthanthatofthepureneuralstemcellgroupine

7、verytimeduringischemia；ThenumberofCaspase‘’3positivecellsintheMKgroupislowerthanthatofthepureneuralstemcellgroupineverytimeduringischemiaTheshorteriStimeofischemia，themoredifferentisthedateConclusions：ist，TheMKlOng／mlist