上皮性鈣粘附蛋白和β-連環(huán)素的表達與膽囊腺癌預(yù)后的關(guān)系.pdf

1、山西醫(yī)科大學碩士學位論文上皮性鈣粘附蛋白和β連環(huán)素的表達與膽囊腺癌預(yù)后的關(guān)系姓名：臧長海申請學位級別：碩士專業(yè)：免疫指導教師：李連青2003.6.15AstudyofEcadherinandpcateninexpressionandtheircorrelationwithprognosisofadenocarcinomaofthegallbladderZANGChanghaiPublishingHouseofChineseJournal

2、otRheumatologyTaiyuan030013，China【Abstract】ObjectiveToinvestigatetheproteinandmRNAexpressionofE—eadherinandBcatenininadenoc—arcinomaofthegallbladderandtofindtheircorrelationwithdifferentiation。metastasisTNMstagingandprog

3、nosisMethodsHigh—sensitiveSPimmunohistoche—micalmethodandinsituhybridizationwereusedtodetecttheproteinandmRNAexpressionofEcadherinand6cateninResultsImmunohistochemistryrevealedthatamongthe60cases，thepositiveratesofE—cadh

4、erinand日一cateninwere40％and45％respectivelyTheabnormalexpressionratesofthesetwoproteinswere583％and633％respec—tivelyTherewasnosignificantrelationshipbetweenE—cadherinandp—cateninstainingandhist0109icaltypeofthetumor(P005)Ho

5、wev—er，therewasastatisticallysignificantdifferencebetweenwellandmod—eratelydifferentiatedcellsandpoorlydifferentiatedcells(P005)IncaseswithlymphaticmetastasistheabnormalexpressionratesofE—cad—herinandp—cateninweresignifi

6、cantlyhigherthanthoseinnonmetastaticcases(PO05)ThemeansurvivaItimeincaseswithabnormalEcadherinandp—cateninexpressionwassignificantlyshorterthanthatincaseswiththeexpressiongrading()～(卅)Insituhybridizationshowedthatinadeno

7、carcinomaofthegallbladder，thepositiverateofE—cadherinand8cateninmRNAwas60％and40％respectivelyTheirconcordancerateswith()～(卅)proteinexpressionwere788％and824％，re—spectivelyConclusionTheconcordancerateofE—cadherinand8catenin

8、mRNAandproteinexpressionwasrelativelyhighTheycanbeusedasmarkersforprognosingadenocarcinomaofgallbladderinclinicalpractice【KeywordsJAdenoearcinoma，gallbladder；Ecadherin；B。catenin；Prognosis；Immunohistochemistry；insituHybri