普通小麥-百薩偃麥草異染色體系分子標記分析及其耐鹽性鑒定.pdf

1、南京農業(yè)大學碩士學位論文普通小麥百薩偃麥草異染色體系分子標記分析及其耐鹽性鑒定姓名：陳華鋒申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：亓增軍20060801ABSTRACTMolecularmarkeranalysisandsalinitytolerancescreenonTriticumaestivumLThinopyrumbessarabicumLSvealienchromosomelinesABSTRACTTotransfert

2、hesalinitytolerancegene(s)ofThinopyrumbessarabicumL6vetoTriticumaestivumL，fivedisomicadditionsandfourtranslocationsofTriticumaestivumcvChineseSpringThbessarabicumwereanalysedbyhighmolecularweightr：uteninsubunits(HMWGS)，s

3、equencetaggedsites(STS)ofbarleyandwheatrestrictionfragmentlengthpolymorphism(RFLP)ThespecificHMWGSofThinopyrumbessarabicumcoutdbeusedtotracechfomosemeIJandIJdisomicadditionwasdevelopedandidentifiedbyGISHSTSmarkersMWG2303

4、(2rm)，7AAG(2BL)，NAU／xiba015(6S)andRFLPprobeBCDl35(2L)couldbeusedtotagThinopyrumbessarabicumchromosomesaddedinCH05andCHlltheresultsofthesemarkersalsevealedthiscb舢osomemighthaveinvolvedrearrngementbetween2Jand6JSTSmarkersM

5、wG974(3HS)BCD269(3HL)andRFLPprobePSRll6(3L)couldbeusedtotracethechromo∞mesaddedinCH09，whichmightbegroupedinto3JSTSmarkerMWG808(7HE)andRFLPprobePSRl63(4L)wereusedtobacethechromosomesaddedinCH03，CH04andCH34，whichmighthavei

6、nvolvedrearrangementbetween4Jand7JSTSnlarker8MWG939(4HL)，ABG472(4HL)MWG522(5HL)，ABG712(5HL)，＆uHg(2AL)andRFLPprobeCD0669(4S)，WGl14(4L)，PSRIS4(6L)allhadaspecificamplificationorhybridizationinThinopyrumbessarabic蝴genomewhil

7、enotargetDNAbandwerefoundinthetestedalienchromosomelinesThesemarkerscouldbeusedtosctfecnnowadditionsinvolvedJehromosom島differentfromthepresenttinesAnewSTSmarker111b01tracingch∞mos伽c3JofThinopyrurabessarabicumWasaslodevel

8、opedSalinitytolerancescrc∞atgenninatinnandseedlingstageconfirmedthatCSThbessarabicumamphiploidconferredtolerancesignificantlyd主強刪fromChin讎SpringThbessarabicumchromosom囂addedinwheatcouldelthancosalinitytoleranceofwheatInt