基于凝血酶與腺苷核酸適配體功能化納米探針識(shí)別的分析方法研究.pdf

1、江南大學(xué)碩士學(xué)位論文基于凝血酶與腺苷核酸適配體功能化納米探針識(shí)別的分析方法研究姓名：彭曉麗申請學(xué)位級(jí)別：碩士專業(yè)：營養(yǎng)與食品衛(wèi)生學(xué)指導(dǎo)教師：樂國偉王周平20080801AbstractNanoprobetechniqueandaptamerrecognitionarenovelprobetechniqueandmolecularsrecognitionmodedevelopingrapidlyinrecentyears，whichhav

2、epresentedtremendouslypotentialintheapplicationofbiologicalanalysis，drugtesting，foodsafetydetection，clinicalverify，environmentalanalysisandtheothersLuminescnectanalysiswiththemeritsofhilghsensitivityandwidelinearrangehas

3、alsoattractedmoreandmoreattentionsrecentlyThispapermeanstoconstructnovelnanoprobesusingthrombinandadenosinebindingwiththeirspecificaptamer—functionalizedAunanoparticles，combining謝t11chemiluminescencedetectingtechnology，t

4、heAuandAgstainingsignalamplificationtechnologyandresonancelightscatteringtechnology，establishmethodssystemssuitforhighlysensitivedetectionofthrombinandadenosineInthemeanwhile，electrochemiluminesceceanalysiswascombinedwit

5、IlcapillaryelectrophoresisseparationtoestablisharapidlyandsensitivelyanalyticalmethodforclarithromycindeterminationinbiologicalfluidsAllthesewouldbeofimportanceforenrichingthetheoryandacceleratingtheapplicationsofnano—pr

6、obeandluminescentanalysistechniqueFirstly，combiningtheaptamerfunctionalizedAunanoparticleswithaptamerrecognitiontechnology，usingthrombinasthemodelanalyte，anovelanalyticalmethodforthrombinWaSdevelopedcoupling、Ⅳithflowinje

7、ctionnanogoldstrippingchemiluminescenceanalysistechniqueThedectionglimitofthismethodis03x10。10egmLSecondly，based011abovereseachusingthrombinasmodelanalyte，introducingitsspecificaptamerrecognisethrombin，thrombinspecificap

8、tamerfunctionalizedAunano—probewereaSsembledtothesolidmatrixsurfaceThenaselectivegolddepositionprocedureongoldnanoparticlessurfacewascarriedouttorealizethefirstsignalamplificationStrippingchemiluminescencedetectionWascon

9、ductedtofurtheramplifythedetectionsignals，makingthesensitivityofthebiologicalsensingsystemenhancedgreatlyThirdly，consideringtheproblemoftimeconsumptioningoldstainsimilartogoldstainingbaSedstrippingCLdetection，ChapterIVse

10、lectivelydepositesilveronthesurfaceofnanogoldforthefnstinsitusignalamplification，andthenbondwiththestrippingchemiluminescencedetectiontocarryoutthesecondsignalamplification，establishedanovelultratracedetectionmethodforth

11、rombinThedectionglimitofthismethodis03xlO‘12g／mLFinally，couplingnanoprobetechnology，aptamerrecognitionwithresonancelightscatteringanalysis，throughrecognisingreactionbetweenadenosineanditsspecificaptamerinhomogeneousmatri

12、x，causingthedeaggregationoftheaggregatednetworkstructureofadenosinespecificaptamerfunctionalizednanogoldstructure，asharplychangeontheresonancelightscatteringsignalcanbeobtainedBasedonit，anovelhomogeneousaptamerrecognizin