頭頸腫瘤患者外周血中循環(huán)DNAP16基因異常甲基化的臨床意義.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文頭頸腫瘤患者外周血中循環(huán)DNAP16基因異常甲基化的臨床意義姓名：康毅申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：腫瘤學(xué)指導(dǎo)教師：米玉錄20070507山西醫(yī)科大學(xué)磺士學(xué)位論文Detectiononaberrantmethylationofpl6geneintheserumofheadandneckneoplasmspatientsABSTRACTobjeetive：Toexploreaberrantmethylationofp16

2、geneintheserumofheadandneckneoplasmspatients，andtostudytheassociationoftheaberrantmethylationstatusofp16genewithsomeclinicalfactors，includingneoplasmmetastasis，tufterlypesMaterialstMethods：Theperipheralbloodof82casesofhe

3、adandneckneoplasmspatientsWaSwithdrawn(19casesofdonors部controls)，allofthesamplewerefinaldiagnosisbypathologicaldiagnosis，thetotalofthemalignantturnoutWas46，thetotalofthebenigntumororbenignlesionWaS36，eachsampleWasdetecte

4、dbymethylationspecificPCR(MSP)ResuRs：Theaberrantmethylationrateofp16geneinheadandneckneoplasmsserumWaS598％(49／82)，andthatindonorsserumWas為53％(1／19)，Therewassignificantdifferencebetweenthem0o05)Theaberrantmethylafionrateo

5、fp16geneinbenigntumororbenignlesionWas278％(10／36)，andthatinmalignanttumourWas848％(39／46)，Therew勰significantdifferencebetweenthemQO05)；Theaberrantmethylationrateofp16ganeinthyroidpapillarycarcinomaamongthethyroiddiseaseWa

6、S917％(22／24)Theaberrantmethylationrateofp16geneinnodulargoiterwas267％(8／30)ThereWaSsignificantdifferencebetweenthem①O05)Conclusion：1Theaberrantmethylationofp16genebetweenheadandneckneoplasmssa岫anddonorsserumhavesignifica

7、ntdifference，itissuggestedthattheaberrantmethylationofp16genedetectedbyMSPcouldreconnaissancehighriskgroupofheadandneckneoplasms；2Theaberrantmethylationofpl6genebetweanhcadandneckbenigntumororbenignlesionandmalignantRlln

8、oor’Selumhavesignificantdifference，itissuggestedthattheaberrantmethylationofp16genedetectedbyMSPcouldprovidlaboratory’evidencefordiscriminateheadandneckneoplaSmspatientsinclinic；3Theaberrantmethylationofp16genebetweenthy

9、roidpapillarycarcinomaandnodulargoiteritissuggestedthattheaberrantmethyhfionofp16genedetectedbyMSPcouldprejudgememthethyroidpapillaryc村cinomaandthenodulargoiterbeforeoperation。Keywords：p16gene，Headandneckneoplasms，Methyl