P-,25-攜帶的玉米抗南方型銹病相關(guān)基因的克隆與定位.pdf

1、j，王106882分類號密級，，I曰蘆學(xué)大等碩士學(xué)位論文單位代碼：10019學(xué)號：S040055P：。攜帶的玉米抗南方型銹病相關(guān)基因的克隆與定位CloningandMappingofRelativeResistanceGenetoSouthernCornRustofP25inMaize研究生：張亙?nèi)笇?dǎo)教師：王室友熬援申請學(xué)位門類級別：盛堂亟專業(yè)名稱：佳塑遣伎直獨(dú)研究方向：垂卷盆王直挫所在學(xué)院：壅堂皇生物技苤堂筮二零零七年六月Abstr

2、aetBasedOnthepreviousresearchofourlab，threerelativesequencesWelreamplifiedfromthegenomieDNAofP25(immuneinbredline)andF349(suseeptibleinbredline)SCRPandSCRP3WCIItderivedfromresistantlineP2，，andFfromsusceptiblelineF349，whi

3、challhadfulllengthopenreadingframe(OPr9encodin93339bl、2655bp、2583bpnueleotidewimdeduced1112，884、860aminoneidSCqUellclsrespectivelyTheconserveddomainNBARCincludedPloop、Kinase2a、Kinase3amotifwasfotmdinthetwotelative懈i吼erI∞

4、geneSCRPandSERP3Therewefeadeletionof11aminoacidatthebeginningofthe5’regionandapointmutationinthePloopmotifcomparingwithRplfamilyandEThecomparisonalsoindicatedsignificantdiffer∞ceof3’regionbetweenthetwogenesandllplfamilyT

5、herewerenoapparentLRRregionsandsequenceiaentitytoRplfa口milyofthe3regionAdeletionoffWaSfoundinlargescllhcomparingwithSCRPandSCRP3ItWaSproposedthatthevariationofNtermintts、therecombinationOi“vailatiOnofCterminUS、theinterac

6、tionofNterminuswithCterminuswereattributedtotheresistancetosouthemtomrustBasedOilthedeletiontwonewnlallrmPISAandPJwereclevelopexlOntheshortmnofehromosome10whichcouldbeusedingenemappingandMASTherewere166individualsofBCIpo

7、pulationofP2，F(xiàn)349investigatedandidentifiedinHainanprovinceThe代釉Itswere1istedOSfollows：thedis島讎infestedinsusceptiblelinewassoutherncornrustaccordingtothethesymptomsandmorphologyofpathogeny；thesegregationratioofBClpopulati

8、onWaS1：1inaccordancewithMendelianmonogcnesegregationlaw；simplesequence喇m“SsR)analysisWaScarriedoutinB(21populationandtheresistantgeneSCRP2jWaSmapped011theshortalmofchromosome10withageneticdistanceofl25eMtoPhill8and162eMt

9、oumel318respectively；thetwonewm棚^Je鵡sharedthes鋤eresultanditwasrestrictedintothelinkagemapwithageneticdistanceof18eM佃$CRP25finallyThefunctionofthetwogenesandtherelationshipbetWtllthetwogenesandresistancetoSoutherncornrust