六個品種羊微衛(wèi)星多態(tài)性的研究.pdf

1、黑龍江八一農墾大學碩士學位論文六個品種羊微衛(wèi)星多態(tài)性的研究姓名：潘振亮申請學位級別：碩士專業(yè)：動物營養(yǎng)與飼料科學指導教師：耿忠誠20070601Abs仃actInthisexperimentthegeneticdiversitiesofNortheastfinewoolsheep(NE)，Australiafinewoolsheep(AnGermanym耐nosheep(GM3，Caudulacoldsheep(CD)HornlessD

2、orsetDown㈣andLiaoningCashmeregoat州)WereevaluatedbysixmierosatellitemarkersitesThehereditaryconstitutionsofsixvarietalssheepwereanalyzedbycalculatingthedegreeofgeneticheterozygosis，polymorphisminformationcontentF—statisti

3、cinspection，geneticcoefficientofdifferentiationgeneticdistanceandsystemclusteranalysis，andtheassoeiativityoflambingWasanalyzedbyusingGCIOI，Bms2508andLsev043micrusatelitesitesatfifthchromosomeFindingasfollowing：IAtsixmicr

4、osatellitesitesofGDF9andBMPl5gene，thereweredetectedtotletinyallelasandthenumberofaverageeffectivealleleswas317Itshowedthattheseintravarietalgeneticvadationeswereplentiful2Thepolymorphisminformationcontentmdifferentmicros

5、atellitesitesoffivesheepcolonyandCashmeregoatweregenerallyhigherTheaveragepolymorphisminformationcontentofNortheastfinewoolsheepWaSO5152，LianningCaShmeregoatWas05029，CaudulacoldsheepWaS05869，AustraliafinewoolsheepWaS0597

6、7，GermanymerinosheepWasO5190，andHornlessDorsetDownWasO5038AmongofthemAustraliafinewoolsheepWaSthemaximumandLiaoningCashmeregoatWastheminimumTheaveragedegreeofheterozygosisofNortheastfinewoolsheepWaS07147。LiaoningCaShmere

7、goatWasO6274，CaudulacoldsheepWasO8063，AustraliafinewoolsheepWaS08998，GermanymerinosheepWasO8723，andHomlessDorsetDownWaSO8644AmongofthemAustraliafinewoolsheepWasthemaximumandLiaoningCaShmeregoatWastheminimumItshowedthatth

8、esixmicrosatellitesitesa：tGDF9andBMPl5ganeofsixvarietieshadaffluentpolymorphism3ThegeneticdistanceofeachvarietyWaSworkedoutbythewayofNeistandardgeneticdistanceTheminimalNeistandardgeneticdistanceamongthemWasbetweenNorthe

9、astfinewoolsheepandAustraliafinewoolsheepandthegeneticdistanceWaSO031595ThemaximalNeistandardgeneticdistanceamongthemWasbetweenNortheaStfinewoolsheepandLiaoningCaShmeregoatandthegeneticdistanceWaSO1045914Thetotallambingo

10、fLiaoningCashmeregoatandNortheastfinewoolsheepof238／200ganutypeatGCl01seatofmicrosatellitesite117／107genotype砒Lsev043seatand154／154genotypeatBms2508seatwerenotablyhigherthanothergenotypes’，whichcouldberegardedaslinkagege