Omi-HtrA2在肝癌細(xì)胞凋亡基因網(wǎng)絡(luò)中對p53基因與XIAP基因調(diào)控作用的實(shí)驗(yàn)研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文Omi/HtrA2在肝癌細(xì)胞凋亡基因網(wǎng)絡(luò)中對p53基因與XIAP基因調(diào)控作用的實(shí)驗(yàn)研究姓名：余丹申請學(xué)位級別：碩士專業(yè)：內(nèi)科學(xué)（消化系?。┲笇?dǎo)教師：林菊生20090401華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 4The role of Omi/HtrA2 in the regulation of p53 gene and the XIAP gene in he

2、patocarcinoma cell apoptosis gene network Institute of Liver Disease, Tongji Hospital, Tongji Medical College, Huazhong Universtiy of Science and Technology Postgraduate: Yu Dan Tutor: Lin Jusheng Abstract 【Objective】

3、The serine protein kinase Omi/HtrA2 is a recently discovered mitochondrial pro-apoptotic factor, and its role in tumor apoptosis is just recognized, but there is still no report about its involvement of live cancer. In t

4、his experiment, by observing the protein expression changes after treating with specific Omi/HtrA2 inhibitor ucf-101, we studied the regulation mechanism of serine protein kinase Omi/HrA2 in p53 gene promoted apoptosis a

5、nd XIAP gene inhibited apoptosis in hepatocarcinoma cells, and explored the possibility that it was a new target to liver cancer gene therapy. 【Methods】 Cultivating the live cell lines L02 and the three hepatocarcinoma c

6、ell lines in vitro (HepG2, Hep3B, Huh7) in which the expression of p53 protein is different: HepG2(p53-wt), Huh7 (p53-mut), Hep3B (p53-null). Administration exogenous therapeutic dose of Omi/HtrA2 inhibitor ucf-101, we d

7、etected the expression of p53, XIAP and Omi/HrA2 gene in the level of protein by western blotting before and after receiving specific inhibitor ucf-101 in liver cancer cell lines HepG2, Hep3B and Huh7and liver cells L02.

8、 【Results】 In the four cell lines in which the expression of p53 protein is different, the expression of the XIAP protein and Omi/HtrA2 protein are also different. The exprssion of Omi/HtrA2 protein in liver cells is hig

9、her than that in normal fetal liver cells L02, and the exprssion in Huh7 cells (p53-mut) is higher than in other hepatoma carcinoma cell lines. The XIAP protein expression was no significant difference in the three hepat