高富集生物活性硒啤酒酵母的研究.pdf

1、山東輕工業(yè)學(xué)院碩士學(xué)位論文高富集生物活性硒啤酒酵母的研究姓名：孫海云申請學(xué)位級別：碩士專業(yè)：發(fā)酵工程指導(dǎo)教師：于同立20080610山東輕T業(yè)學(xué)院碩lj學(xué)位論文ABSTRACTSeleniumiSoneofthenecessaryelementstomaintainthenormallireactivityofhuman’Sbody。Theuseofselenizedyeastasenrichedseleniumsupplementsi

2、nhumannutritionhasbecomeatopicofincreasinginterestoverthelastdecadeThissubjectusedthebeeryeastoflaboratoryastheoriginalstrains，strainswerefilteredfortheresistancetotheSeleniumandoptimizedthecultureconditionsoftheSerich，a

3、ndbyaseriesoftrainingmethodsmadetheabilityofseleniumrichedhadagreatincrease，suchashandledofthecellwallusingacomplexenzyme(snail—enzyme)，ultrasoundtreatmentinthecellmembraneandincreasedpressureinthecultureprocess，anditlai

4、dagoodfoundationforthefurtherappliedtoindustrialproduction。ThethreebrewersyeaststrainswerefilteredfortheresistancetotheSeleniumwiththemethodofflat—panelcolonycountingThroughdetectedtheirbiomassandseleniumintakerateinthem

5、ediumcontainingthesodiumselenitesolution，andthestrain362khadhigherbiomassandresistancetothesodiumseleniteTheexperimentalconditionsofthemethodfordetectingtheselo玨i疆l秘————≮hereductionofmethyleneblueWasimprovedthroughthesin

6、glefactorandorthogonalexperiment，andtheresultshowed：Asthelightwasweak，thereactiontemperaturewas32“CandformaldehydeaddedquantitywaslmL，thereactionspeedWasappropriatetotime，lineargood，andsensitivitywashighTheSe—richedcultu

7、reconditionsforthestrain362kwereoptimizedthroughsinglefactorandorthogonalexperimentsandconfirmeditsoptimalcultureconditions：thetemperaturewas28℃，theconcentrationofsodiumseleniteaddedwasbatchto20；tg／mL，thevaccinationwas10

8、％(v／v)，runningspeedwas200r／min，andculturedfor48h，the362k’Sgrowthconditions，biomassandSe—enrichmentcapacitywasbest。Inthisoptimalconditions，seleniumyeastbiomass(dryweight)couldupto84869／L，thetotalseleniumcontentreached7064

9、2399／gdrycell，includingorganicseleniumwas6429161tg／gdrycell，theconversionratereached9l。01percentItcouldbeshowedthatthebeeryeast’Sabilityoftransformingtheinorganicseleniumtoorganicselinumwasstrongthroughtheoptimizationofc

10、ultureconditions，ithadreachedmorethan80percentSotheseleniumintakebecameacriticalfactorofseleniumenrichedrateInthisissueused搬ethreemethodstoincreasetheintakeofseleniumfollowing：thetreatmentcellwallwithcompoundenzyme，ultra