基質(zhì)金屬蛋白酶-2基因啟動子區(qū)異常甲基化與喉鱗狀細胞癌關(guān)系研究.pdf

1、華中科技大學碩士學位論文基質(zhì)金屬蛋白酶-2基因啟動子區(qū)異常甲基化與喉鱗狀細胞癌關(guān)系研究姓名：翟亮申請學位級別：碩士專業(yè)：耳鼻咽喉科學指導教師：孔維佳20090501華 中 科 技 大 學 碩 士 學 位 論 文 3The research of relationship between the aberrant methylation of matrix metalloproteinase-2 promoter and laryngea

2、l squamous carcinoma Abstract Objectives: To explore the relationship between the aberrant methylation of matrix metalloproteinase-2 (MMP-2) gene promoter and the progression of laryngeal squamous carcinoma (LSCC). Metho

3、ds: We carried out a detailed methylation analysis of the MMP-2 promoter and studied the level of MMP-2 mRNA and protein expression in 40 biopsy samples isolated from patients with LSCC and 5 normal laryngeal membrane sa

4、mples by bisulfite sequence-PCR (BSP), RT-PCR, Western-blot. The correlation analysis was used to research the relationship between the hypomethylation of MMP-2 promoter and its mRNA, protein expression and TNM stages of

5、 LSCC. Results: Methyl Primer Express software displayed the promoter site of MMP-2 gene showed abundant CpG islands. The MMP-2 mRNA and protein expressions in each TNM stage of LSCC samples had up-regulation in differen

6、t level (P<0.01). Bisulfite sequence-PCR (BSP) showed the hypomethylation status of MMP-2 promoter in LSCC samples. The hypomethylation levels were associated with MMP-2 gene expressions, and the differences of hypome

7、thylation levels in advanced TNM stages and advanced lymph node status were significant (P<0.01). Conclusions: The hypomethylation of MMP-2 promoter in LSCC relates to its high expression. Hypomethylation of MMP-2 pro