轉(zhuǎn)化生長因子β2體外抑制牛角膜內(nèi)皮細(xì)胞增殖的分子研究.pdf

1、華中科技大學(xué)碩士學(xué)位論文轉(zhuǎn)化生長因子β2體外抑制牛角膜內(nèi)皮細(xì)胞增殖的分子研究姓名：陸秀蘭申請學(xué)位級別：碩士專業(yè)：眼科學(xué)指導(dǎo)教師：張明昌20090501華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 華 中 科 技 大 學(xué) 碩 士 學(xué) 位 論 文 3Molecular mechanism of TGF-β2's inhibitory effect on bovine corneal endothelial cells in

2、vitro Department of Ophthalmology,Union hospital, Tongji Medical College, Huazhong University of Science and Technology Candidate: Xiulan Lu Supervisor: Professor Mingchang Zhang Abstract 【Objective】 To obs

3、erve the inhibitory effect of TGF-β2 on the bovine corneal endothelial cells(bCEC) and explore its possible molecular mechanism. 【Material and Methods】 Part1: MTT assay was applied to detect the inhibitory effect of a v

4、ariety of concentrations of TGF-β2 on the bCECs.Flow cytometer was used to measure the cell cycle of different concentration groups 48h after the treatment. Part 2: The bCECs were cultured in vitro and incubated in a var

5、iety of concentrations of TGF-β2 for 48h,then collected for RNA extraction and reserve transcriptionThe primer was designed for p27Kip1 based on its gene sequence. The expression of gene and protein p27Kip1 was detected

6、by RT-PCR and Western blotting. 【Result】 The 0.1 and 1ng/L group represented significant difference compared with the negative control group and the other groups in the same testing time. After 48h's incubation,the 0

7、.1 and the 1ng/L group represented the significant differences of theG0/G1 cells rate with the negative control group The 1ng/L group represent the significant difference with the 10 and the 100ng/L group, while there wa