An Analysis of the Hormones,Antioxidant Enzymes Activities under Drought Stress and GenomiC-Wide Analysis of NAC Genes Family i.pdf

1、Soybean (Glycine max L.Merr.)is oneof the most prominent legume crops playingsignificant role in world's food security.Water stress is one of the mostsevere constraintson crop production.Present study investigated the ef

2、fects of drought stress imposed forvarious periods on antioxidant activities and hormones biosynthesis in (drought treated)
　　and control (non-drought treated), fourSoybeans (Glycine max L.Merr.) cultivars viz.(jindou

3、 74,jindou 78) as droughttolerant and (H228, B217) as drought sensitive.Considerable variations in the behavior of antioxidants were observed under droughtstress in all Soybeans cultivars.Exposure of plant to drought str

4、ess considerably increaseMDA concentrations in leaves of all cultivars, and such an increment was more indrought sensitive cultivars.The ROS were scavenged by the enhanced activities ofenzymatic (SOD, CAT,POD and APX) an

5、tioxidants inresponse to drought stress withthe passage of time; therefore, maximum values for these attributes were observed at 8
　　days of drought for all cultivars.Theseantioxidant activities were higher in drought

6、tolerant cultivars as compared todrought-sensitive cultivars at various time points.Moreover, Activities of hormones (ABA,IAA,JA and SA) biosynthesis were enhancedwith the passage of time.Data depicted that drought stres

7、s substantially affected thehormone levels inall Soybean cultivars.Variations were apparent among cultivarsregarding theirresponse to drought stress.The (jindou 74,jindou 78) drought tolerantcultivarsremained superior to

8、 sensitive cultivars regarding hormone levels andantioxidantactivities.In summary, our results are suggested that manipulation of these
　　antioxidants and hormones may lead to improvement in drought stress tolerance.<

9、br>　　Generally, one of the largest groups of plant-specific transcription factorsis NACsproteins,[(NAM) no apical meristem,[ATAF1/2] Arabidopsis transcription activationfactor and (CUC2) cup-shaped cotyledon] which play

10、a vital role inplant growth,development and adaption to the environmental conditions.Due to multidimensional roleof NAC proteins,itis very import to identify and characterize this proteins family insoybean.In present stu

11、dy, 139 Gin.NACs genes were identified and analyzed,phylogenetictreewas constructed, genes structures,genome localizations, domainduplications andgenes expression summaries in soybean.The phylogenic analysisrevealed that

12、 theNACs genes could be categorized broadlyinto 18 groups.Thechromosomal localizations and genome duplication indicated139 Gm.NACs contained747 homologues and distributed across 20 chromosomes.There were similar genesstr

13、uctures in conformity with Gm.NACs groups between 190 and678 in proteinsequences lengths (average length 362.7).The motifs composition of Gin.NACs washighly conserved within the same group.Most of the significantly affec

14、ted genes bydrought were concentrated on chromosome 6 based on transcdptomic changes evaluationby digital geneexpression (DGE)in hairy roots.Furthermore,Gm.NAC005,Gm.NAC020,Gm.NAC070 and Gm.NAC117 demonstrated significan

15、tlyhigherexpression changes under drought treatments via quantitative real-timePCR(RT-qPCR)analyses.Taken together, 139 NACs genes in Soybean wereidentifiedwith a carefulinvestigation of their location,structure, duplica

16、tion, and evolution.Likewise, somegroups have evolved,resulting in high levels of functional divergence.Interestingly,Gm.NACs genes respond differently to drought stress which indicates the importance of thisgene family