高脂血癥對(duì)局灶性腦缺血大鼠腦組織神經(jīng)元型一氧化氮合酶（nNOS）表達(dá)的影響.pdf

1、分類寸密級(jí)902953單位代碼學(xué)巴101142003202高脂血癥對(duì)局灶性腦缺血大鼠腦組織神經(jīng)元型一氧化氮合酶(nNOS)表達(dá)的影響研冗生：苤建些指導(dǎo)教帥廑登蓮』：』￡醫(yī)壓申請(qǐng)學(xué)仲專業(yè)研究所在J類級(jí)別：名稱：方向：學(xué)院：醫(yī)堂亟主韭壘塹堂盟魚暨瘤一笠二l魚壓醫(yī)堂睡西醫(yī)科人學(xué)第臨床醫(yī)學(xué)院2006年4月17HAbstractObjective：Inthisstudy，weexaminetheexpressionofnNOSafterfocal

2、cerebralischemiaandreperfusioninjuryinratswithhyperlipidemia，observetheinfarctionvolumeandthemorphologicchange，investigatedtherelationshipbetweennNOSandneuronaldamageMethods：Thel60wistarratswererandomlydividedintotwogrou

3、ps：group1werefedwithmormalforage；group2werefedwithhighfatforageAfterthehyperlipemicratsweremade，eachgroupwasdividedintosixgroup：sham—operatedgroup，simpleischemia4hourf4h)，ischemia4handreperfused2h，，4h，6h，12hafterthemiddl

4、ecerebralarteryocclusion(MCAO)MCAOwasestablishedbyinsertingnylonthreadcoatedwithparaffinthroughtheintemalcarotidartery(ECA)intotheanteriorcerebralarteryfACA)Thenreperfusionwasallowedfordifferenttimeasmentionedabovebefore

5、decapitationInshamoperatedcontrolrats，thethreadwasonly12millimeterloneAftertheMCAOwasmade，eachofthe6groupwasdividcdintotowgroupsgroupl：ImmunocytochemistrytwasperformedtodeterminethelevelofnNOSgroup2：Theinfarctionvolumewa

6、sobservedby23，5一triphenyltetrazoliurnfTTClstainThecontentsofnitricoxide(NO)inbrainwereexaminedwithnitricacidereducetasemethodResults：(1)MCAOestablishedbyinsertingnylonthreadcoatedwithparaffinisagoodischemiaandreperfusion

7、model(2)Bothhyperlipemicratsandnothyperlipemicratshavethemaximuminfarctionvolumeintheischemia4handreperfused12hgroupInthesameinfarctionmadreperfusiontimegroup，theinfarctionvolmneofhyperlipemicratsislargerthanthenormallip

8、idrats’(3)BothhyperlipemicratsandnonnallipidratshavethemaximumexpressionofnNOSintheischemia4handreperfused12hgroupInthesameinfarctionandreperfusiontimegroup，theexpressionofnNOSofhyperlipemicratsismorethanthenormallipidra