新型根管充填劑——復(fù)方牡蠣粉的生物相容性的研究.pdf

1、青島大學(xué)碩士學(xué)位論文新型根管充填劑——復(fù)方牡蠣粉的生物相容性的研究姓名：劉夢冬申請學(xué)位級別：碩士專業(yè)：口腔臨床醫(yī)學(xué)指導(dǎo)教師：鄧婧鐘德鈺20060513BiocompatibilityStudyofCompositeOysterSheHPowerasaNewRootCanalFillingMaterialPostgraduatestudentLiuMengdongDirectedbyDengJingAbstract【objective[T

2、0evaluatethebiocompatiblityofnewrootcanal衄咄material[Methods[Accordingtotheevaluatedcriteriaofmedicalmaterials，boilogyandanimaltrialsrecommendedinISO10993，theeastersofpowderpartandthemixtureofthenewmaterialwerepreparedsep

3、aratelyandselectedasthetesLTheV79cellwasusedaathetestcellThenewmaterialwasusedasthetestcontrolandthePhenolasthepositivecontr01TheIV[ITassayWasemployedjnOUrstudytoevaluatecytotoxiceffectInthemictDnucleustestinvitro(MtCr)t

4、hemitomycinC(MMC)WasusedasthepositivecontrolthenumberofmicronueleusWasUsedasthecriterialforthedetectionofgenotoxicityTherootcanalKIlingmaterialwasimplantedintothesubcutaneoustissueofratsGeneralandhistochemicalexamination

5、sOfperipheralpackedtissueofmaterialswereperformedatdifferenttimesIntheskinsensitisanfiontestthemaximizationmethodwasselected(Magnusso&Kligman)o9％physiologicalsalline’：5％formaldehydesolutionandtheextractionofmaterialwerei

6、njectedrespectiveHyintodermisofgnineapigon17thdayaftercompletionoftopicalinductionphase，challengeallthetestsandcontrolanimalswiththeextracts，andobservetheerythemaoredemaappearanceofthechallengeskinsitesat24h，48hand72h，an

7、dcalculatesensitizationrate[Results]The舯valuesintestgroupsandnegativegroupswerenotsignificantlydifferentatdifferenttlmes(P005)Thenumberofmicronueleusjntestgroups(powdergroup；complexgroup)wassimilartothenegativecontrol，wh

8、iletheyweresigmfiesnflydifferenttothepositivecontrol(PO05)PathologicaltissuesectionshowedthattheperipheralpackedtissueofmaterialsandtheinflammationchangeaccordedwiththegeneralregularityofinflammatoryoutcomeTheskinsensiti

9、zationofthenewmaterialwasnotapparent[Conclusion】皿enewrootcanalfillingmaterialshowdnodetectedcytotoxicgenomxiceffect，potentialofskinsensitizationandto#cairritationandhasgoodbiocompatibilityKeywords：rootcanalfillingmateria