商品育肥豬黏膜抗體的檢測(cè)及分析.pdf

1、吉林農(nóng)業(yè)大學(xué)碩士學(xué)位論文商品育肥豬黏膜抗體的檢測(cè)及分析姓名：魏林林申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：預(yù)防獸醫(yī)學(xué)指導(dǎo)教師：何昭陽(yáng)20080601DetectionandAnalysisofCommodityPigMucosaAntibodyAbstractMISisgenerallydistributedoverairtube，enteron，mucosaofGenitourinary，andsomeadenoidtissueofanacrineg

2、land，ismainplaceofspecificimmunityfunctionsIgAofmucosaimmuneresponseprocessismajoreffectfactorofmucosaimmuneMucosatissueisalargeexternalenvkonmentsurface，isaplacewhereanimalorganismandmilieuexterieurexchangeeachother，muc

3、osaisdefensivebarrierbetweenanimalorganismandmilieuexterieurasaMucosalimmunesystemorlocalimmunesystem，especiallyimmunologicalactivityingredientswhichishappenedinairtubeenteron，Genitourinarytractandalsomuchmucosa，fordefen

4、ceeffectoforganisminfectedismoreandmorereconstrtopeopleThistextthroughthedetectthedifferentpartsofcommoditypig，suchasoralcavitystomach，duodenum，jejunum，ileum，appendix，colon，rectum，rlose，windpipe，bronchus，lung，bladder，uri

5、ne，COrllUuteri，bodyofuterus，neckofuterus，vaginatearfluid，bile，paristhmic7一globulin，thecontentoflgA、IgG、IgM，teflecttopographyoforganmucosaantibodythewholearticlecontainthreepart，aSfollowing：Part1：overviewthesystemandcompo

6、sitionofMucosalimmunityprocessofimlTluneresponseresearchadvancement，mucosaimmuneanddiseaSehaveintimatecorrelation，theresearchofMucosalimmunesystemnotonlyincreasethenewcontentfortraditionarybutalsoofferthethinkingandmetho

7、dofresearchaboutpathopoiesismechanismofmanydisease，therelationshipofetiologicalagentandhost，diseasediagnosenewgenerationvaccineandprecautionpraeparatumPart2：theestablishmentofmucosaantibodydetectionmethod，samplinganddete

8、ctionfromtheestablishmentofELISAtonowalreadygraduallymature，ELISACandetectantigen，alsoCandetectantibodythistestpreparemouseagainstswine7globulinandrabbitagainstswineyglobulin，theantibodyofIgA、IgG、IgM，usehorseradishperoxi

9、dase；horseradishperoxidase(HRP)tolabelingtosetupthemethodofdoubleantibodysandwichELISA，establishdouble—antibodysandwichELISAtodetectantibodyTodetecteachcollectivemucosaposition(eachpositioncollect30sampleproduct)Part3：de