野生番茄感染葉霉菌后生化性狀的變化及其抗病機制的初步研究.pdf

1、東北農(nóng)業(yè)大學碩士學位論文野生番茄感染葉霉菌后生化性狀的變化及其抗病機制的初步研究姓名：呂曉梅申請學位級別：碩士專業(yè)：蔬菜學指導教師：李景富2001.5.1ABSTRACT碩士學位論文東北農(nóng)業(yè)大學StudyonBiochemicalCharactersandResistanceMechanismofTomatoWildSpeciesInfectedbyCladosporium戶lvumCookeAbstractTomatoleafmold

2、causedbycladarpornonfulvwnCookeoftentakesplacesduringthetomatoproductionofprotectivefield.Tomatowildspecieswereusedtosolvethisproblemintomatobreeding.Thisexperimentwascomposedoffourparts.Firstlytomatoleafmoldfromtheplast

3、ichousesoflocalareawasstudiedbyartificialsprayinoculationconsultingN.HubbelingsphysiologicracediferentialhostsandadoptingPDaysclassificationsystem.Theresultsindic&Mthattherewerethreeracesinlocalareaincludingrace12.3口and3

4、.Secondlytheseedl噸resistancetoleafmoldofeighttomatowildspecieswereidentifiedbyinoculatingwithCladarporiwnfuhamrrace12.3thatisdominantinourregion.Theresultshowedthatallthesetomatowildspecieswereresistanttorace123.Thirdlyb

5、asedonthisresultwestudieddynamicchangesofbiochemicalcharacters(PODPPOrelativelyconductivityraclignincontent)inleavesofthesewildspeciesinoculatedafterld3d5d8dlldl5dtofindbiochemicalmechanismoftomatowildspeciesresistanceto

6、Cla:farpariwnfuMon.Afterinoculationgenerallytheperoxidase(POD)orpolyphenoloxidase(PPO)activitiesinleavesoftestedtomatowildspeciesincreasedsignificantlyincomparisonwiththoseoftheircontrolplants.Relativelyconductivityratei

7、nleavessignificantlyincreasedespeciallyatIstand3rddayafterinoculation.An氏lignincontentintestedwildspeciesleavesafterinoculationalsoincreasedthanthoseofthecontrolplants.Finallytransmissionandscanningelectronmicroscope~use

8、dtostudyleavesoftomatowildspeciesinfected勿Cladosporlwn孫amn.Thefungalgrowthontheleavesoftomatowildspecieswascompletelyarrested妙wildhost15daysafterinoculationAlsothecellwallsofleavesoftestedwillspecieswerethickerthanthoseo

9、fsusce如blevariety(L402).AllthesemadetomatowildspeciesresistingtoCladosporirmrfulvwn.Postgraduate:LvxiaomeiMajor:VegetableBreedingAdvisor.Prof.LijingfuKeywordsL)roperskmesculennonwildtomatospeciesCadosponurn.燦aanCookebioc