基于分子鑒定的水稻DH群體的建立及其應(yīng)用研究.pdf

1、中國農(nóng)業(yè)科學(xué)院碩士學(xué)位論文基于分子鑒定的水稻DH群體的建立及其應(yīng)用研究姓名：葉勝海申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：農(nóng)業(yè)推廣作物學(xué)指導(dǎo)教師：楊長(zhǎng)登張小明20091201AbstractDoubledhaploids(DH)obtainedfromantherculturehavebeenrecognizedasavaluabletoolinplantbreedingItoffersthequickestmethodofadvancingheter

2、ozygouslinestohomozygosityWiththedevelopmentofmolecularbiology，DHpopulationhasbeenwidelyappliedtogenemappingandfunctionalgenomicsresearchesforitsefficiencyandspeedinessHybridofjaponica／indicacombinationZhonghua11／Zhongzu

3、14wasconstructedandantherculturedofthehybridwascarriedout112diploidregenerantplantswereobminedTheDHpopulationWasfurtheranalyzedforitsdistortedsegregationingenotypeandcharactersandcorrelationanalysisofcookingandnutrientqu

4、alitieswerecarriedoutResultsareasfollows：1748calliwereobtainedfromanthercultureofFiofZhonghua11／Zhongzu14154greenregenerantsweregrownfromdifferentiationofthecalliTherefore，callirateoftheantherculturewas172％Greenregeneran

5、tsrateWas2059％Haploidandmultiploidplantswereabandonedfiomtheregenerantplants112doublehaploidplantswereobtainedwithdoublehaploidrateof7273％2Alloftheregenerantplantsweredistinguishedhomozygousbyusing22polymorphicmarkersIts

6、uggestedthattheregenerantplantspopulationwasaccuratelyobtainedfrommicrosporeThe22markerswereusedtoanalyzedistortedsegregationoftheobtainedpopulationingenotypeResultsshowedthatitwasincontinuoussegregationandshowednormaldi

7、stributionTheprojectindicatedthattheDHpopulationwasconstructedaccuratelywithmicrosporederivedregenerantsandnormalsegregationbyusingSSRpolymorphicmarkers，andwhichcouldbeefficientlycarriedoutearlierbyusingSSRmarkers3Riceam

8、ylosecontent(AC)，gelconsistence(GC)，alkalinespreadingvalue(ASV)，proteincontent(PC)and17kindsofaminoacidcontentoftheDHpopulationconstructedfromjaponicafemaleparentZhonghua11andindicamaleparentZhongzu14weremeasuredandcorre

9、lationbetweentheseindexeswereanalyzedstatisticallyContinuousvarianceswererevealedforthe21parametersintheDHpopulationandtransgressivewereshowedformostoftheparametersResultsofcorrelationanalysisindicatedthatanytwoofthethre

10、ecookingparameters(AC，GCandASV)weresignificantlynegmivecorrelationNosignificantcorrelationwasrevealedbetweenPCandACbutpositivecorrelationbetweenPCandASVandnegativecorrelationbetweenPCandGCwerefoundCertaincorrelationcould

11、befoundbetweenthethreecookingparametersandsomekindsofaminoacidcontenLsuchasnegativecorrelationbetweenACand10kindsofaminoacidcontent，negativecorrelationbetweenGCand12kindsofaminoacidcontent，positivecorrelationbetweenASVan

12、dallkindsofaminoacidcontentexceptcysteinePositivecorrelationsbetweenPCandallthe17kindsofaminoacidcontentweredemonstratedwithabove05ofcorrelationcoefficientexceptthatbetweenPCandmethionineorcysteineKeyword：rice，DHpopulati