慢性腎衰動物模型制備方法及大黃游離蒽醌的藥效學研究.pdf

1、成都中醫(yī)藥大學碩士學位論文慢性腎衰動物模型制備方法及大黃游離蒽醌的藥效學研究姓名：楊鵬申請學位級別：碩士專業(yè)：中西醫(yī)結(jié)合指導教師：李明權2007成都中醫(yī)藥大學碩士學位論文3AbstractObjective:WedesignedfiveSDratmodelsofchronicrenalfailure(CRF)whichisadenineadministrated34nephrectomy56nephrectomyligationtheu

2、pdownpolesofunilateralkidneyligationtheupdownpolesofunilateralkidneyrefrigerationtheoutsidectexofthesamekidney.Contrastedtothefiveratmodelswestudytheirstability.wepreliminaryinvestigatethepotencyofnomadicanthraquinoneofR

3、hubarbtotheCRF.Methods:SDratswereromlydividedintosixgroups:onenmalgroupfivemodelgroupsmodelⅠgroup(adeninelavaged)modelⅡgroup(34nephrectomy)modelⅢgroup(56nephrectomy)modelⅣgroup(ligationtheupdownpolesofunilateralkidney)mo

4、delⅤgroup(ligationtheupdownpolesofunilateralkidneyrefrigerationtheoutsidectexofthesamekidney).Foureightweeksafterprocedureweobservedgeneralconditionweightmtalityofratstheureanitrogencreatininecontentofratserum.Wecollecte

5、dthe56nephrectomyCRFmodeladministratednomadicanthraquinoneofRhubarbtothemodelratsindertoobservegeneralconditionweightofratstheureanitrogencreatininecontentofserumthepathologicalchangeofresidualnephridium.Results:Wecompar

6、edwiththemodelgroupsthenmalgroup1.Ratweightofmodelgroupsdecreased(P0.05)；2.Theureanitrogenthecreatininecontentofmodelratserumraised(P0.01)；3.AfterfoureightweeksprocedureratsmtalityofmodelⅢgroupreducedthanothermodelgroups

7、；4.WecomparedwiththetreatmentgroupsthemodelgroupafterfourweeksadministrattionwithnomadicanthraquinoneofRhubarb.Intreatmentgroupstheureanitrogencreatininecontentofratserumweredecreased(P0.01或0.05)；thenumberofrenaltubulesg