喜樹熱激反應(yīng)的分子生態(tài)學(xué)研究.pdf

1、東北林業(yè)大學(xué)博士學(xué)位論文喜樹熱激反應(yīng)的分子生態(tài)學(xué)研究姓名：于景華申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：植物學(xué)指導(dǎo)教師：祖元?jiǎng)偭鴧⒖?0030601ABSTRACTCamptothecaacuminataisatreeuniquetoChina，producingterpenoidindolealkaloidCrL～)10一Hydroxycamptothecin0=|CPT)andeamptothecin(cPD，whichpossessstronga

2、ntiCancerbioactivityThemolecularecologyofheatshocktoCamptothecaacuminateseedlingswiththetheoryandmethodoffunctionalproteomieswassystematicallystudiedBythestudyweattemptedtoelucidatethemolecularmechanismofecologicaladapta

3、tionandevolvingofplanttoheatshockinthemethodofphysiologicalecologymolecularbiologyofplants。TheresearchmeasuredthephotosyntheticpotentialchangeswhentheCamptothecaacuminataseedlingsweretreatedbyheatshockatthesametimethephy

4、siologicindexdynamicchangesweremeasured酗followsifheywereMDA、chlorophyllandso011WealsomeasuredthemetabolizedchangesaboutthetwoimportantalkaloidsofCamptothecaacuminataUnderthedifferenttreatedconditionsdifferentialbandswere

5、obtainedthroughthedenaturedgelelectrophoresis，thenmainlyaccordingtothe2DElectrophoresis，thedotsappearedbytheeoomassiebrilliantbluedying，thus11dotswereseparatedOfthesedots6including7kindsofproteinswereprimarilyidentifiedi

6、ntermofthepeptidequalityfingerprintingmapThen，5kindsofthe7proteinswerediagnosedontheirecologicalacclimationfunctionduringtheheatshockresponsebythebiologicalinformaticsThestudydiscussedthemolecularmechanismonheatshockmeta

7、bolismregulationofcamptothecinsOnthisbasecombinedwiththeproteindata，theecologicalaccfimationofCamptothecaacuminatatoheatshockresponsewasanalyzedThenweconcludedthemolecularecologicalmechanismofCamptothecaacuminatatoheatsh

8、ockresponseSimultaneouslyweusedtheCamptothecaacuminataheatstressasmodeprobedintothepossiblemechanismonCamptothecaacuminatadroughtresistancemodulatedbyeamptothecinmetabelismKeywords：Camptothecaacuminata；heatshockresponse；