甘藍(lán)型油菜PolCMS恢復(fù)基因的分子標(biāo)記開發(fā)與候選BAC分析.pdf

1、卿辛十震幸天李HUAZHONGAGRICUIJ，URALUNIVERSITY碩士學(xué)位論文MASTER’SDEGREEDISSERTATION甘藍(lán)型油菜PolCMS恢復(fù)基因的分子標(biāo)記開發(fā)與候選BAC分析MOLECULARMARKERDEVELOPMENTOF‘poLIMA，CMSFERTILITYRESToRERGENE扭萬尸)ANDITSCANDIDATEBACCLONESANALYSISIN丑丑通召國了〔減八只P〔ISL.研究生:CA

2、NDIDATE:劉念LIUNIAN導(dǎo)師:楊光圣教授SUPERVISOR:PROFESSORYANGGUANGSHENG專業(yè):作物遺傳育種MAJOR:CROPGENETICSANDBREEDING研究方向:FIELD:油菜分子生物學(xué)MOLECULARBIOLOGYONRAPESEED中國武漢WUHAN，CHINA二O一O年五月MAY，2010華中農(nóng)業(yè)大學(xué)2010屆碩士學(xué)位論文目錄摘要............................

3、........................................……，.................................................……IABSTRACT..................................................................................................................……H

4、縮略詞表.....................................................................................................................……nl1文獻(xiàn)綜述....................................................................................……，...

5、……，....……，二，，，.，.41.1油菜雜種優(yōu)勢(shì)的利用.......................................……41.2細(xì)胞質(zhì)雄性不育與恢復(fù)基因的研究..................................................................……41.2.1細(xì)胞質(zhì)雄性不育.................................................

6、......................……、.............……51.2.2植物細(xì)胞質(zhì)雄性不育育性恢復(fù)基因研究...................................................……61.2.3油菜波里馬細(xì)胞質(zhì)雄性不育恢復(fù)基因的研究及應(yīng)用...............................……81.3植物分子標(biāo)記開發(fā)研究進(jìn)展及其運(yùn)用.......................

7、.......................................……91.3.1利用數(shù)據(jù)庫序列和生物信息學(xué)手段開發(fā)新的分子標(biāo)記.........................……101.3.2利用分子生物學(xué)實(shí)驗(yàn)技術(shù)開發(fā)新的分子標(biāo)記及運(yùn)用.............................……121.4植物基因分離中的圖位克隆技術(shù)............................................

8、........一141.4.1圖位克隆的技術(shù)環(huán)節(jié).................................................................................……141.4.2圖位克隆技術(shù)存在的問題.........................................................................……161.5本研究的目的和意義.....

9、.............................一162材料與方法.............................................................................................................……182.1實(shí)驗(yàn)材料..............................................................

10、.............................................……182.2標(biāo)記的開發(fā)與驗(yàn)證...........................................................................................……182.2.1序列對(duì)比與標(biāo)記的開發(fā)..................................................

11、..........................……182.2.2DNA混合池的構(gòu)建...................................……‘二，......................................……182.2.3驗(yàn)證SCAR標(biāo)記...........……，...................................................................

12、...……192.3交換單株的鑒定...............................................................................................……192.3.1單株葉片樣品DNA的提取.....................................................................……192.3.2交換單株的檢測與遺

13、傳圖譜的繪制........................................................……202.3.3恢復(fù)基因標(biāo)記通用性檢測........................................................................……202.4與恢復(fù)基因相關(guān)的BAC克隆的PCR鑒定分析.........................……，..........