Molecular epidemiology and transmission features of Colistin resistance genes(mcr).pdf

1、分類號(hào)UDC學(xué)號(hào)密級(jí)埸H1大譬YANGZHOUUNlVERSlTY博士學(xué)位論文DHl300lMolecularepidemiologyandtransmissionfeaturesofColistinresistancegenes(mcr)指導(dǎo)教師姓名申請(qǐng)學(xué)位級(jí)別論文提交日期學(xué)位授予單位AfrahKamaIAbdElazimYassin至盛塑鏨蕉：揚(yáng)劌盤堂：望苤揚(yáng)劌：2至多QQ壁：一譴學(xué)科專業(yè)名稱：塑墮?？锾茫?Q12生!Q旦論文答辯日

2、期：2Q12生12旦：揚(yáng)劌壟鱟學(xué)位授予日期：2Q12生12旦答辯委員會(huì)主席：工壁嬰窒亟20l7年12月㈧4洲6洲4㈣5mⅢm7，洲33ⅢYPhDD1ssertatmn2017(MDR)wasmostcommoninisolatesfromducks(44／44，100％)，followedbychickens(568／644，882％)，pigs(93／113，823％)andCOWS(13／61，213％)2Wholegenomese

3、quenceofselectedEscherichiacoliisolatesNineteenchnicalEcohisolatescollectedfromchicken，pig，COWandduck，wereselectedforthewholegenomicsequencingbasedontheirphenotypingbackgroundofantimicroblalresistanceGenomlcDNAwaspurifie

4、dfromallisolatesandsequencedaspaired—endreadsontheIlluminaplatformThewebserversCentreofGenomicEpidemiologywasusedtoanalyzethewholegenomesequenceandidentifyantibioticresistancegenesandvirulencefactorsOverall，the789％ofisol

5、atescarried29differentresistancegenesconferringresistanceto12classesofantlmicrobialsAllthe19EcoltstainscarriedmorethanoneresistancegeneOnlyoneisolate(52％)caniedraCY一，geneinfull—lengthcopyofacohstlnresistancegeneThirteent

6、ypesofplasnudrepliconsin15／19(789％)isolateswererecognized3Epidemiolugyofmcr1geneinChinaToinvestigatethepresenceofthecolistinresistancegene(mcr—J)InChina，wedevelopedtwoqPCRs(mcr—JqPCR，total—bacteriaqPCR)todeterminedirectl

7、ytheprevalenceofthetact‘一andthetotalbacteriainsamplesThemCF一』waspositivewithpoultrysamplesfrom21ofthe25provincesThehighestprevalencewasnasalandanalswabsfrompigs(812％；1，128／1，389)followedbyfecalswabsfromdogs(350％；14／40)，o

8、ro—pharyngealandcloacalswabsfrompoultry(271％；899／3，318)，vaginalswabsfromwomen(O7％，1／134)，andbovinefecalsamples(0％；0／64)Theratiosofthemcr—Jandthetotal—bacteriacop)numberswere316％withfecalswabsfromdogs，17％withcloacaland04％

9、withoro—pharyngealswabsfrompoultry，O1％withnasalandanalswabsfrompigsandO0008％withvaginalswabsfromwomenThel卅lCl。一Jlevelsdidnotdiffersignificantlybetweennasalandanalswabsinpigs，andbetweenoro—pharyngealandcloacalswabsinpoult