一株甜菜夜蛾核型多角體病毒某些生物學(xué)特性的研究.pdf

1、華中師范大學(xué)碩士學(xué)位論文一株甜菜夜蛾核型多角體病毒某些生物學(xué)特性的研究姓名：蘇越申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：洪華珠2000.6.1StudiesOnSomeBiologicalCharacteristicsofSpodopteraexiguaNuclearPolyhedrosisVirusAbstractTIlisthesisreportedtheresearchresultsofthemorphologyobservat

2、ion，hostrail【gedeterminationandinvivobioassayofSpodopteraexiguanucle81“polyhedrosisvirusAmericanisolate111ecomparativestudiesonthetoxityandrestrictionendonucleaseanalysisofSeNPVAmeficanisolateandZhongShmlUnlversityisolat

3、ewerealSeportedScreenedwiththeelectronmicroscope也eextractofthespodopteraexiguanuclearpolyhedrosisviruspesticidewerepurepolyhedraandSeNPVAmeficanisolarwasmultiplenuclearpolyhedrosisvirusnlethirdinstarlarvaefromfourdiffere

4、ntkindofinsects，SpodopteraexisuaHeliothisarmiseraSpodopteralituraandPktellaxylos據(jù)llawerefedwithSeNPVAmericanisolateattheconcentrationof1310’PIB“1111n圯resultshowedth砒onlySelarvaewereseriouslyinfectedanddiedfourdayslaterth

5、eothersremainedalive，fromWhichwecoulddrawaconclusion吐lattheScNPVhashighspecifichostrangeinviVOThe碡suItsofinviVObioassayindicatedthatthcLC50ofSeNPVAmericanisolatetothirdinstarfourthinstarandfiRhinstarSelarvaewas125910PIBs

6、lml。6466XlO’鷹s加!11and152010“Pmstmlrespectively丁hirdinstarSe】a1VaeweminfectedwithSeNPVAmedcanisolateattheconcentrationof1310‘P113s／ml，l3103PtBs／ml，1310Pmaml，13l仃，PIBs／m1and13X106PIBs／rrd，andtheLT∞Was8柏days641days，525days4

7、99daysand428daysrespecdvelyThcfourthinsial“andfi觸instarSelarvaewerebothinfectedattheconcentrationof13103Pms，IIll。1310Pms／ml，13l礦PⅢs，fnl，13X106PIBslmland1310’Pms，ml。theLTsotofourthinstarSelaryaewas882days798days。782days，6

8、37daysand577daysrespectivelyWhiletheLTsotofifminstarSelarvaewas939days，870days，818days，769daysand733daysrespectivelyTheresultsshowedthatwhenvirusconcentrationandtemperaturewerefixed。thesensitivityWasreducedaslarvaeinstar